Sea cucumbers in a high temperature and low dissolved oxygen world: Roles of miRNAs in the regulation of environmental stresses

doi:10.1016/j.envpol.2020.115509

	Sea cucumbers in a high temperature and low dissolved oxygen world: Roles of miRNAs in the regulation of environmental stresses
	Huo, Da 1,2,3,4,6; Sun, Lina 1,2,3,4,6; Sun, Jingchun1,2,3,4,6 ; Zhang, Libin 1,2,3,4,6; Liu, Shilin 1,2,3,4,6; Su, Fang 1,2,3,4,6; Yang, Hongsheng1,2,3,4,5,6
	2021
发表期刊	ENVIRONMENTAL POLLUTION
ISSN	0269-7491
卷号	268 页码:9
通讯作者	Sun, Lina(sunlina@qdio.ac.cn)
摘要	The exacerbation of global warming has driven changes in environmental factors, including water temperature and oxygen concentration. The sea cucumber Apostichopus japonicus, an economically important aquatic animal, is constantly and directly challenged by heat and hypoxia. In this study, 12 small RNA libraries were constructed for this species, and a total of 21, 26 and 22 differentially expressed (DE) miRNAs were clarified in A. japonicus under thermal (26 degrees C), hypoxic (2 mg/L) and the combined stresses. Comparative miRNA sequencing analysis and real-time PCR were used to identify and validate the representative miRNAs, including Aja-miR-novel-299, Aja-let-7b-3p, Aja-miR-71b-5p, Aja-miRnovel-13218 and Aja-miR-2004 in response to high temperature, and Aja-miR-92b-3p, Aja-miR-210-5p and Aja-miR-novel-26331 in response to oxygen limitation. GO and KEGG pathway analysis revealed that the potential target genes of DE-miRNAs involved in biosynthesis, metabolism, immunity, cell growth and death, translation and signaling transduction. Key DE-miRNAs with potentially targeted genes associated with heat shock and hypoxia response were also determined. These results may help explaining the role of miRNA regulation in stress resistance, as well as the potential molecular regulation mechanism of the echinoderm A. japonicus in the context of global warming. (C) 2020 Elsevier Ltd. All rights reserved.
关键词	Global climate change MicroRNA Apostichopus japonicus Anoxia Heat
DOI	10.1016/j.envpol.2020.115509
收录类别	SCI
语种	英语
资助项目	Strategic Priority Reseach Program of the Chinese Academy of Sciences[XDA24030304] ; National Natural Science Foundation of China[41776161] ; National Natural Science Foundation of China[41776162] ; Agricultural Seed Project of Shandong Province[2017LZGC010] ; International Partners Program of Chinese Academy of Sciences[133137KYSB20180069] ; Taishan Scholars Program (Distinguished Taishan Scholars) ; Youth Innovation Promotion Association CAS[2019209]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000600553000102
出版者	ELSEVIER SCI LTD
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169924
专题	海洋生态与环境科学重点实验室
通讯作者	Sun, Lina
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Cent China Div, Wuhan 430071, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Huo, Da,Sun, Lina,Sun, Jingchun,et al. Sea cucumbers in a high temperature and low dissolved oxygen world: Roles of miRNAs in the regulation of environmental stresses[J]. ENVIRONMENTAL POLLUTION,2021,268:9.
APA	Huo, Da.,Sun, Lina.,Sun, Jingchun.,Zhang, Libin.,Liu, Shilin.,...&Yang, Hongsheng.(2021).Sea cucumbers in a high temperature and low dissolved oxygen world: Roles of miRNAs in the regulation of environmental stresses.ENVIRONMENTAL POLLUTION,268,9.
MLA	Huo, Da,et al."Sea cucumbers in a high temperature and low dissolved oxygen world: Roles of miRNAs in the regulation of environmental stresses".ENVIRONMENTAL POLLUTION 268(2021):9.

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