Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
A Fish Galectin-8 Possesses Direct Bactericidal Activity | |
Zhang, Tengfei1,2,3; Jiang, Shuai1,2,3; Sun, Li1,2,3 | |
2021 | |
发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES |
卷号 | 22期号:1页码:15 |
通讯作者 | Jiang, Shuai(sjiang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn) |
摘要 | Galectins are a family of animal lectins with high affinity for beta-galactosides. Galectins are able to bind to bacteria, and a few mammalian galectins are known to kill the bound bacteria. In fish, no galectins with direct bactericidal effect have been reported. In the present study, we identified and characterized a tandem repeat galectin-8 from tongue sole Cynoglossus semilaevis (designated CsGal-8). CsGal-8 possesses conserved carbohydrate recognition domains (CRDs), as well as the conserved HXNPR and WGXEE motifs that are critical for carbohydrate binding. CsGal-8 was constitutively expressed in nine tissues of tongue sole and up-regulated in kidney, spleen, and blood by bacterial challenge. When expressed in HeLa cells, CsGal-8 protein was detected both in the cytoplasm and in the micro-vesicles secreted from the cells. Recombinant CsGal-8 (rCsGal-8) bound to lactose and other carbohydrates in a dose dependent manner. rCsGal-8 bound to a wide range of gram-positive and gram-negative bacteria and was co-localized with the bound bacteria in animal cells. Lactose, fructose, galactose, and trehalose effectively blocked the interactions between rCsGal-8 and different bacteria. Furthermore, rCsGal-8 exerted potent bactericidal activity against some gram-negative bacterial pathogens by directly damaging the membrane and structure of the pathogens. Taken together, these results indicate that CsGal-8 likely plays an important role in the immune defense against some bacterial pathogens by direct bacterial interaction and killing. |
关键词 | galectin-8 Cynoglossus semilaevis bacterial binding bactericidal immune defense |
DOI | 10.3390/ijms22010376 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key Research and Development Program of China[2018YFD0900500] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
WOS记录号 | WOS:000606289800001 |
出版者 | MDPI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/169975 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Jiang, Shuai; Sun, Li |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Zhang, Tengfei,Jiang, Shuai,Sun, Li. A Fish Galectin-8 Possesses Direct Bactericidal Activity[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(1):15. |
APA | Zhang, Tengfei,Jiang, Shuai,&Sun, Li.(2021).A Fish Galectin-8 Possesses Direct Bactericidal Activity.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(1),15. |
MLA | Zhang, Tengfei,et al."A Fish Galectin-8 Possesses Direct Bactericidal Activity".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.1(2021):15. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
ijms-22-00376-v2.pdf(6336KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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