Characterization of the first cultured free-living representative of Candidatus Izemoplasma uncovers its unique biology

doi:10.1038/s41396-021-00961-7

	Characterization of the first cultured free-living representative of Candidatus Izemoplasma uncovers its unique biology
	Zheng, Rikuan 1,2,3,4,5; Liu, Rui 1,2,3,5; Shan, Yeqi 1,2,3,4,5; Cai, Ruining 1,2,3,4,5; Liu, Ge 1,2,3,5; Sun, Chaomin 1,2,3,5
	2021-03-21
发表期刊	ISME JOURNAL
ISSN	1751-7362
页码	16
通讯作者	Sun, Chaomin(sunchaomin@qdio.ac.cn)
摘要	Candidatus Izemoplasma, an intermediate in the reductive evolution from Firmicutes to Mollicutes, was proposed to represent a novel class of free-living wall-less bacteria within the phylum Tenericutes. Unfortunately, the paucity of pure cultures has limited further insights into their physiological and metabolic features as well as ecological roles. Here, we report the first successful isolation of an Izemoplasma representative from the deep-sea methane seep, strain zrk13, using a DNA degradation-driven method given Izemoplasma's prominent DNA-degradation potentials. We further present a detailed description of the physiological, genomic and metabolic traits of the novel strain, which allows for the first time the reconstruction of the metabolic potential and lifestyle of a member of the tentatively defined Candidatus Izemoplasma. On the basis of the description of strain zrk13, the novel species and genus Xianfuyuplasma coldseepsis is proposed. Using a combined biochemical and transcriptomic method, we further show the supplement of organic matter, thiosulfate or bacterial genomic DNA could evidently promote the growth of strain zrk13. In particular, strain zrk13 could degrade and utilize the extracellular DNA for growth in both laboraterial and deep-sea conditions. Moreover, the predicted genes determining DNA-degradation broadly distribute in the genomes of other Izemoplasma members. Given that extracellular DNA is a particularly crucial phosphorus as well as nitrogen and carbon source for microorganisms in the seafloor, Izemoplasma bacteria are thought to be important contributors to the biogeochemical cycling in the deep ocean.
DOI	10.1038/s41396-021-00961-7
收录类别	SCI
语种	英语
资助项目	Major Research Plan of the National Natural Science Foundation[92051107] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; Key Deployment Projects of Center of Ocean Mega-Science of the Chinese Academy of Sciences[COMS2020Q04] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; National Key R and D Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc] ; Open Research Project of National Major Science & Technology Infrastructure (RV KEXUE)[NMSTI-KEXUE2017K01]
WOS研究方向	Environmental Sciences & Ecology ; Microbiology
WOS类目	Ecology ; Microbiology
WOS记录号	WOS:000631006000004
出版者	SPRINGERNATURE
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170136
专题	深海极端环境与生命过程研究中心
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所; 深海极端环境与生命过程研究中心; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 深海极端环境与生命过程研究中心; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zheng, Rikuan,Liu, Rui,Shan, Yeqi,et al. Characterization of the first cultured free-living representative of Candidatus Izemoplasma uncovers its unique biology[J]. ISME JOURNAL,2021:16.
APA	Zheng, Rikuan,Liu, Rui,Shan, Yeqi,Cai, Ruining,Liu, Ge,&Sun, Chaomin.(2021).Characterization of the first cultured free-living representative of Candidatus Izemoplasma uncovers its unique biology.ISME JOURNAL,16.
MLA	Zheng, Rikuan,et al."Characterization of the first cultured free-living representative of Candidatus Izemoplasma uncovers its unique biology".ISME JOURNAL (2021):16.

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