Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha

doi:10.1111/jfb.14722

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha
	Qiu, Reng 1,2,3; Sun, Yuan Y.4; Guan, Cui C.3; Kan, Yun C.1,2,3,5; Yao, Lun G.1,2,3
	2021-04-24
发表期刊	JOURNAL OF FISH BIOLOGY
ISSN	0022-1112
页码	9
通讯作者	Kan, Yun C.(kanyunchao@163.com) ; Yao, Lun G.(lunguangyao@163.com)
摘要	T lymphocytes play an important role in cellular and adaptive immunity in vertebrates. The mechanisms of the fish immune system are little studied because of the lack of population-specific antibodies. This study examined the expression of two T lymphocyte markers, TCR alpha (PoTCR alpha) and CD8 alpha (PoCD8 alpha) in the Japanese flounder (Paralichthys olivaceus). The expression of PoTCR alpha and PoCD8 alpha was mainly detected in immune/mucosal tissues. Recombinant PoTCR alpha and PoCD8 alpha were expressed in pET32a and pET259, respectively. Then, rabbit anti-PoTCR alpha serum and rat anti-PoCD8 alpha serum were prepared. Using serum, the characteristics of TCR+ and CD8+ head kidney leucocytes (HKLs) were investigated. The results of laser scanning confocal microscopy (LSCM) demonstrated that TCR alpha and CD8 alpha were transmembrane proteins localized on the cell surface. The populations of CD8 alpha(-), CD8 alpha(+), TCR alpha(-), and TCR alpha(+) were sorted by flow cytometry (FCM) and analysed using qRT-PCR. The results demonstrated that all TCR alpha(+)/TCR alpha(-) or CD8 alpha(+)/CD8 alpha(-) HKLs expressed IFN-gamma. The CD4-1 and IgM transcripts were detected only in TCR alpha(-) and CD8 alpha(-) cells. Furthermore, HKL mitogenesis was induced with concanavalin A (ConA) stimulation. Taken together, the results from LSCM and FCM analyses showed that mammalian and P. olivaceus TCR+ and CD8(+) leucocytes share basic characteristics.
关键词	antiserum CD8α head kidney leucocytes Paralichthys olivaceus TCRα
DOI	10.1111/jfb.14722
收录类别	SCI
语种	英语
资助项目	Doctoral Research Project of Nanyang Normal University[ZX2014060] ; Henan Provincial Scientific and Technological Research Project[202102110109] ; National Natural Science Foundation of China[31870917] ; Program for Innovative Research Team of Science and Technology in University of Henan Province[20IRTSTHN024]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000643157000001
出版者	WILEY
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170604
专题	实验海洋生物学重点实验室
通讯作者	Kan, Yun C.; Yao, Lun G.
作者单位	1.Nanyang Normal Univ, Coll Life Sci & Agr Engn, China UKNYNU RRes Joint Lab Insect Biol, Nanyang 473061, Peoples R China 2.Nanyang Normal Univ, Coll Life Sci & Agr Engn, Henan Prov Engn & Technol Ctr Hlth Prod Livestock, Nanyang 473061, Peoples R China 3.Nanyang Normal Univ, Henan Key Lab Ecol Secur Water Source Reg Mid Lin, Nanyang 473061, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 5.Henan Univ, Sch Life Sci, Kaifeng 475000, Peoples R China
推荐引用方式 GB/T 7714	Qiu, Reng,Sun, Yuan Y.,Guan, Cui C.,et al. Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha[J]. JOURNAL OF FISH BIOLOGY,2021:9.
APA	Qiu, Reng,Sun, Yuan Y.,Guan, Cui C.,Kan, Yun C.,&Yao, Lun G..(2021).Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha.JOURNAL OF FISH BIOLOGY,9.
MLA	Qiu, Reng,et al."Characterization of TCR+ and CD8(+) head kidney leucocytes in Japanese flounder (Paralichthys olivaceus) with antisera against TCR alpha and CD8 alpha".JOURNAL OF FISH BIOLOGY (2021):9.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
jfb.14722.pdf（2057KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览