Megalocytivirus Induces Complicated Fish Immune Response at Multiple RNA Levels Involving mRNA, miRNA, and circRNA

doi:10.3390/ijms22063156

IOCAS-IR > 实验海洋生物学重点实验室

	Megalocytivirus Induces Complicated Fish Immune Response at Multiple RNA Levels Involving mRNA, miRNA, and circRNA
	Wu, Qian 1,2,3; Ning, Xianhui 1; Sun, Li 1,2,3
	2021-03-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	22 期号:6 页码:20
通讯作者	Sun, Li(lsun@qdio.ac.cn)
摘要	Megalocytivirus is an important viral pathogen to many farmed fishes, including Japanese flounder (Paralichthys olivaceus). In this study, we examined megalocytivirus-induced RNA responses in the spleen of flounder by high-throughput sequencing and integrative analysis of various RNA-seq data. A total of 1327 microRNAs (miRNAs), including 368 novel miRNAs, were identified, among which, 171 (named DEmiRs) exhibited significantly differential expressions during viral infection in a time-dependent manner. For these DEmiRs, 805 differentially expressed target mRNAs (DETmRs) were predicted, whose expressions not only significantly changed after megalocytivirus infection but were also negatively correlated with their paired DEmiRs. Integrative analysis of immune-related DETmRs and their target DEmiRs identified 12 hub DEmiRs, which, together with their corresponding DETmRs, formed an interaction network containing 84 pairs of DEmiR and DETmR. In addition to DETmRs, 19 DEmiRs were also found to regulate six key immune genes (mRNAs) differentially expressed during megalocytivirus infection, and together they formed a network consisting of 21 interactive miRNA-messenger RNA (mRNA) pairs. Further analysis identified 9434 circular RNAs (circRNAs), 169 of which (named DEcircRs) showed time-specific and significantly altered expressions during megalocytivirus infection. Integrated analysis of the DETmR-DEmiR and DEcircR-DEmiR interactions led to the identification of a group of competing endogenous RNAs (ceRNAs) constituted by interacting triplets of circRNA, miRNA, and mRNA involved in antiviral immunity. Together these results indicate that complicated regulatory networks of different types of non-coding RNAs and coding RNAs are involved in megalocytivirus infection.
关键词	Paralichthys olivaceus megalocytivirus miRNA ceRNA immune response
DOI	10.3390/ijms22063156
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31730100] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000645765500001
出版者	MDPI
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170628
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, CAS Ctr Ocean Mega Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wu, Qian,Ning, Xianhui,Sun, Li. Megalocytivirus Induces Complicated Fish Immune Response at Multiple RNA Levels Involving mRNA, miRNA, and circRNA[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(6):20.
APA	Wu, Qian,Ning, Xianhui,&Sun, Li.(2021).Megalocytivirus Induces Complicated Fish Immune Response at Multiple RNA Levels Involving mRNA, miRNA, and circRNA.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(6),20.
MLA	Wu, Qian,et al."Megalocytivirus Induces Complicated Fish Immune Response at Multiple RNA Levels Involving mRNA, miRNA, and circRNA".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.6(2021):20.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
ijms-22-03156-v2.pdf（2356KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览