A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture

doi:10.1080/21505594.2021.1926649

	A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture
	Zhao, Yan1,2,3,4 ; Jiang, Shuai 2,3,4; Zhang, Jian 2,3,4,5; Guan, Xiao-Lu 2,3,4; Sun, Bo-Guang2,3,4 ; Sun, Li 2,3,4
	2021
发表期刊	VIRULENCE
ISSN	2150-5594
卷号	12 期号:1 页码:1362-1376
通讯作者	Sun, Li(lsun@qdio.ac.cn)
摘要	Recent studies indicate that the Bacillus species is distributed in deep-sea environments. However, no specific studies on deep-sea Bacillus cereus have been documented. In the present work, we isolated a B. cereus strain, H2, from the deep-sea cold seep in South China Sea. We characterized the pathogenic potential of H2 and investigated H2-induced death of different types of cells. We found that H2 was capable of tissue dissemination and causing acute mortality in mice and fish following intraperitoneal/intramuscular injection. In vitro studies revealed that H2 infection of macrophages induced pyroptosis and activation of the NLRP3 inflammasome pathway that contributed partly to cell death. H2 infection activated p38, JNK, and ERK, but only JNK proved to participate in H2-triggered cell death. Reactive oxygen species (ROS) and intracellular Ca2+ were essential to H2-induced activation of JNK and NLRP3 inflammasome. In contrast, lysosomal rupture and cathepsins were required for H2-induced NLRP3 inflammasome activation but not for JNK activation. This study revealed for the first time the virulence characteristics of deep-sea B. cereus and provided new insights into the mechanism of B. cereus infection.
关键词	Bacillus cereus deep-sea cold seep infection NLRP3 inflammasome pyroptosis
DOI	10.1080/21505594.2021.1926649
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences[KLMVI-OP-202002] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Immunology ; Infectious Diseases ; Microbiology
WOS类目	Immunology ; Infectious Diseases ; Microbiology
WOS记录号	WOS:000652027500001
出版者	TAYLOR & FRANCIS INC
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170956
专题	深海极端环境与生命过程研究中心
通讯作者	Sun, Li
作者单位	1.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 5.Chinese Acad Sci, Deep Sea Res Ctr, Inst Oceanol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhao, Yan,Jiang, Shuai,Zhang, Jian,et al. A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture[J]. VIRULENCE,2021,12(1):1362-1376.
APA	Zhao, Yan,Jiang, Shuai,Zhang, Jian,Guan, Xiao-Lu,Sun, Bo-Guang,&Sun, Li.(2021).A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture.VIRULENCE,12(1),1362-1376.
MLA	Zhao, Yan,et al."A virulent Bacillus cereus strain from deep-sea cold seep induces pyroptosis in a manner that involves NLRP3 inflammasome, JNK pathway, and lysosomal rupture".VIRULENCE 12.1(2021):1362-1376.

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