Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform

doi:10.1016/j.bios.2021.113262

	Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform
	Song, Fengge1 ; Wei, Yangdao 1; Wang, Peng 3; Ge, Xiaolin 1; Li, Chaoyang 1; Wang, Aimin 1; Yang, Zhiqing 1; Wan, Yi 1,3,4; Li, Jinghong 2
	2021-08-01
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	185 页码:5
通讯作者	Yang, Zhiqing(yangzhiqing@hainanu.edu.cn) ; Wan, Yi(993602@hainu.edu.cn)
摘要	Nucleic acid-based diagnosis using CRISPR-Cas associated enzymes is essential for rapid infectious disease diagnosis and treatment strategies during a global pandemic. The obstacle has been blossomed CRIPSR-Cas based tools that can monitor wide range of pathogens in clinical samples with ultralow concentrations. Here, a universal nucleic acid magneto-DNA nanoparticle system was exploited for the detection of pathogenic bacteria, based on the collateral cleavage activity of CRISPR-Cas14a and tag-specific primer extension. In the system, the target nucleic acids were amplificated and be separated from mixtures by streptavidin-coated magnetic bead. The collateral cleavage activity of CRISPR-Cas14a can be activated via the tag sequence on the target product. Consequently, the fluorophore quencher reporter can be activated by CRISPR-Cas14a, leading to the increasing response. The exploited universal bacterial diagnostic can distinguish six different bacteria strains with 1 cfu/mL or 1 aM sensitivity, which may provide new strategies to construct fast, accurate, cost-effective and sensitive diagnostic tools in environments with limited resources.
关键词	Bacterial diagnostics CRISPR-Cas14a Tag-specific primer extension Magneto-DNA
DOI	10.1016/j.bios.2021.113262
收录类别	SCI
语种	英语
资助项目	Hainan Provincial Natural Science Foundation of China[420RC518] ; Open Fund of Shandong Key Laboratory of Corrosion Science[KLCS201910] ; Tsinghua University Initiative Scientific Research Program[2020Z99CFZ019] ; Tsinghua University Spring Breeze Fund[2020Z99CFZ019] ; Research Foundation of Hainan University[KYQD (ZR) 1711] ; Research Foundation of Hainan University[KYQD (ZR) 1997] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF04] ; Open fund of Fujian Provincial Key Laboratory of Photonics Technology[JYG 2002]
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000655570200001
出版者	ELSEVIER ADVANCED TECHNOLOGY
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/171002
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Yang, Zhiqing; Wan, Yi
作者单位	1.Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 2.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Bio Fouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Song, Fengge,Wei, Yangdao,Wang, Peng,et al. Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform[J]. BIOSENSORS & BIOELECTRONICS,2021,185:5.
APA	Song, Fengge.,Wei, Yangdao.,Wang, Peng.,Ge, Xiaolin.,Li, Chaoyang.,...&Li, Jinghong.(2021).Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform.BIOSENSORS & BIOELECTRONICS,185,5.
MLA	Song, Fengge,et al."Combining tag-specific primer extension and magneto-DNA system for Cas14a-based universal bacterial diagnostic platform".BIOSENSORS & BIOELECTRONICS 185(2021):5.

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