Screening of genes expressed in vivo after infection by Vibrio anguillarum M3

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	Screening of genes expressed in vivo after infection by Vibrio anguillarum M3
	Zou, Y. -X.; Mo, Z. -L.; Hao, B.; Ye, X. -H.; Guo, D. -S.; Zhang, P. -J.; Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2010
发表期刊	LETTERS IN APPLIED MICROBIOLOGY
ISSN	0266-8254
卷号	51 期号:5 页码:564-569
摘要	Aims: Genes uniquely expressed in vivo may contribute to the overall pathogenicity of an organism and are likely to serve as potential targets for the development of new vaccine. This study aims to screen the genes expressed in vivo after Vibrio anguillarum infection by in vivo-induced antigen technology (IVIAT). Methods and Results: The convalescent-phase sera were obtained from turbot (Scophthalmus maximus) survived after infection by the virulent V. anguillarum M3. The pooled sera were thoroughly adsorbed with M3 cells and Escherichia coli BL21 (DE3) cells. A genomic expression library of M3 was constructed and screened for the identification of immunogenic proteins by colony immunoblot analysis with the adsorbed sera. After three rounds of screening, 19 putative in vivo-induced (ivi) genes were obtained. These ivi genes were catalogued into four functional groups: regulator/signalling, metabolism, biological process and hypothetical proteins. Three ivi genes were insertion-mutated, and the growth and 50% lethal dose (LD50) of these mutants were evaluated. Conclusions: The identification of ivi genes in V. anguillarum M3 sheds light on understanding the bacterial pathogenesis and provides novel targets for the development of new vaccines and diagnostic reagents. Significance and Impact of the Study: To the best of our knowledge, this is the first report describing in vivo-expressed genes of V. anguillarum using IVIAT. The screened ivi genes in this study could be new virulent factors and targets for the development of vaccine, which may have implications for the development of diagnostic regents.
关键词	Virulence Identification Vulnificus Cloning
学科领域	Biotechnology & Applied Microbiology ; Microbiology
URL	查看原文
收录类别	sci
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1753
专题	实验海洋生物学重点实验室
通讯作者	Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zou, Y. -X.,Mo, Z. -L.,Hao, B.,et al. Screening of genes expressed in vivo after infection by Vibrio anguillarum M3[J]. LETTERS IN APPLIED MICROBIOLOGY,2010,51(5):564-569.
APA	Zou, Y. -X..,Mo, Z. -L..,Hao, B..,Ye, X. -H..,Guo, D. -S..,...&Mo, ZL, Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).Screening of genes expressed in vivo after infection by Vibrio anguillarum M3.LETTERS IN APPLIED MICROBIOLOGY,51(5),564-569.
MLA	Zou, Y. -X.,et al."Screening of genes expressed in vivo after infection by Vibrio anguillarum M3".LETTERS IN APPLIED MICROBIOLOGY 51.5(2010):564-569.

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