Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction | |
Wei, Yangdao1; Yang, Zhiqing1; Zong, Chengli1; Wang, Buhua1; Ge, Xiaolin1; Tan, Xiao2; Liu, Xin4; Tao, Zhenzhen1; Wang, Peng3; Ma, Chunxin1; Wan, Yi1,3; Li, Jinghong2 | |
2021-10-04 | |
发表期刊 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION |
ISSN | 1433-7851 |
页码 | 8 |
通讯作者 | Wan, Yi(993602@hainanu.edu.cn) ; Li, Jinghong(jhli@mail.tsinghua.edu.cn) |
摘要 | As a CRISPR-Cas system (clustered regularly interspaced short palindromic repeats and CRISPR associated proteins), Cas14a1 can cis/trans cleave single-stranded DNA (ssDNA). Here, we describe an unreported capacity of Cas14a1: RNA can trigger the trans ssDNA cleavage. This Cas14a1-based RNA-activated detection platform (Amplification, Transcription, Cas14a1-based RNA-activated trans ssDNA cleavage, ATCas-RNA) has an outstanding specificity for the detection of target RNAs with point mutation resolution, which is better than that of the Cas14a1-based ssDNA-activation. Using ATCas-RNA via a fluorophore quencher-labeled ssDNA reporter (FQ), we were able to detect 1 aM pathogenic nucleic acid within 1 h, and achieve 100 % accuracy with 25 milk samples. This platform can serve as a new tool for high-efficiency nucleic acid diagnostics. Importantly, this work can expand our understanding of Cas14a1 and inspire further mechanisms and applications of Class-2 Cas systems. |
关键词 | CRISPR-Cas14a1 DNA cleavage pathogenic bacteria RNA RNA detection platform |
DOI | 10.1002/anie.202110384 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key Research and Development Program of China[2018YFD0900704] ; National Natural Science Foundation of China[21621003] ; National Natural Science Foundation of China[41866002] ; Tsinghua University Initiative Scientific Research Program[2020Z99CFZ019] ; Tsinghua University Spring Breeze Fund[2020Z99CFZ019] ; Research Foundation of Hainan University[KYQD(ZR)1711] ; Research Foundation of Hainan University[KYQD(ZR)1997] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF04] |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000703187000001 |
出版者 | WILEY-V C H VERLAG GMBH |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/176480 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Wan, Yi; Li, Jinghong |
作者单位 | 1.Hainan Univ, Sch Life Sci, Sch Pharmaceut Sci, Marine Coll,State Key Lab Marine Resource Utiliza, Haikou 570228, Hainan, Peoples R China 2.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 4.Univ Shiga Prefecture, Sch Environm Sci, Dept Ecosyst Studies, 2500 Hassaka Cho, Hikone, Shiga 5228533, Japan |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Wei, Yangdao,Yang, Zhiqing,Zong, Chengli,et al. trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2021:8. |
APA | Wei, Yangdao.,Yang, Zhiqing.,Zong, Chengli.,Wang, Buhua.,Ge, Xiaolin.,...&Li, Jinghong.(2021).trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,8. |
MLA | Wei, Yangdao,et al."trans Single-Stranded DNA Cleavage via CRISPR/Cas14a1 Activated by Target RNA without Destruction".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021):8. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
anie.202110384.pdf(2146KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 |
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