Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles | |
Xu, Chaojie1,2,3,4; Xing, Ronge1,2,3; Liu, Song1,2,3; Qin, Yukun1,2,3; Li, Kecheng1,2,3; Yu, Huahua1,2,3; Li, Pengcheng1,2,3 | |
2021-06-30 | |
发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES |
ISSN | 0141-8130 |
卷号 | 181页码:398-409 |
通讯作者 | Li, Pengcheng(pcli@qdio.ac.cn) |
摘要 | In this study, we generated chitosan nanoparticles by exploiting the electrostatic interactions between positively charged hydroxypropyltrimethyl ammonium chloride chitosan (HACC) and negatively charged carboxymethyl chitosan (CMC), and examined the effects of altering the molecular weight and carboxymethyl substitution sites of the chitosan molecules. Particle size, potential, and encapsulation efficiency of the various chitosan nano particles were examined; the particle size range was 162.40 & ndash;332.80 nm, the charge range was 19.50 & ndash;40.60 mV, and the encapsulation efficiency range was 48.4 & ndash;70.7%. We then examined the immunostimulatory effects of the nanoparticle variants on dendritic cells (DCs); we found that the site of carboxymethyl substitution significantly affected the immunostimulatory effects of the nanoparticles. Two nanoparticle types, 200 kDa N,O-carboxymethyl chitosan-HACC (NO-CMC-HACC) and N-carboxymethyl chitosan-HACC (N-CMC-HACC), greatly promoted the expression of interleukin-6, tumor necrosis factor, and interleukin-1 beta in DCs. Moreover, NO-CMC-HACC nanoparticles caused an increase in major histocompatibility complex-II (MHC-II), CD11c, CD80, and CD86 secretion in DCs, indicating that these nanoparticles promoted antigen presentation. We then examined chitosan nanoparticle uptake by DCs using laser confocal microscopy; we found that the NO-CMC-HACC nanoparticles were more readily absorbed by DCs compared to the N-CMC-HACC nanoparticles. Therefore, we concluded that 200 kDa NO-CMCHACC nanoparticles exhibited strong potential as immunological adjuvants. (c) 2021 Elsevier B.V. All rights reserved. |
关键词 | Carboxymethyl chitosan DCS cells Hydroxypropyltrimethyl ammonium chloride chitosan Immunostimulatory activities Nanoparticles |
DOI | 10.1016/j.ijbiomac.2021.03.148 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | National Key Research and Development Program of China[2018YFC0311300] ; Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2019YYSP028] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
WOS记录号 | WOS:000656912700007 |
出版者 | ELSEVIER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/176844 |
专题 | 实验海洋生物学重点实验室 |
通讯作者 | Li, Pengcheng |
作者单位 | 1.Chinese Acad Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
第一作者单位 | 中国科学院海洋大科学研究中心 |
通讯作者单位 | 中国科学院海洋大科学研究中心 |
推荐引用方式 GB/T 7714 | Xu, Chaojie,Xing, Ronge,Liu, Song,et al. The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,181:398-409. |
APA | Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2021).The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,181,398-409. |
MLA | Xu, Chaojie,et al."The immunostimulatory effects of hydroxypropyltrimethyl ammonium chloride chitosan-carboxymethyl chitosan nanoparticles".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 181(2021):398-409. |
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