Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus)

doi:10.1186/s12862-021-01837-2

IOCAS-IR > 实验海洋生物学重点实验室

	Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus)
	Wang, Yunong 1,2,3,4; Zhou, Li 2,3,4; Wu, Lele2,3,4 ; Song, Changbin 5; Ma, Xiaona 2,3; Xu, Shihong 2,3; Du, Tengfei2,3,4 ; Li, Xian 1,2,3; Li, Jun 2,3,4
	2021-06-07
发表期刊	BMC ECOLOGY AND EVOLUTION
卷号	21 期号:1 页码:12
通讯作者	Li, Xian(lixian@ouc.edu.cn)
摘要	BackgroundAs flatfish, turbot undergo metamorphosis as part of their life cycle. In the larval stage, turbot live at the ocean surface, but after metamorphosis they move to deeper water and turn to benthic life. Thus, the light environment differs greatly between life stages. The visual system plays a great role in organic evolution, but reports of the relationship between the visual system and benthic life are rare. In this study, we reported the molecular and evolutionary analysis of opsin genes in turbot, and the heterochronic shifts in opsin expression during development.ResultsOur gene synteny analysis showed that subtype RH2C was not on the same gene cluster as the other four green-sensitive opsin genes (RH2) in turbot. It was translocated to chromosome 8 from chromosome 6. Based on branch-site test and spectral tuning sites analyses, E122Q and M207L substitutions in RH2C, which were found to be under positive selection, are closely related to the blue shift of optimum light sensitivities. And real-time PCR results indicated the dominant opsin gene shifted from red-sensitive (LWS) to RH2B1 during turbot development, which may lead to spectral sensitivity shifts to shorter wavelengths.ConclusionsThis is the first report that RH2C may be an important subtype of green opsin gene that was retained by turbot and possibly other flatfish species during evolution. Moreover, E122Q and M207L substitutions in RH2C may contribute to the survival of turbot in the bluish colored ocean. And heterochronic shifts in opsin expression may be an important strategy for turbot to adapt to benthic life.
关键词	Turbot Benthic life Adaption Opsin Heterochronic shift
DOI	10.1186/s12862-021-01837-2
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation - National Natural Science Foundation of China[41976122] ; National Key R&D Program of China - Ministry of Science and Technology of the People's Republic of China[2017YFB0404000] ; Key R&D Program of Shandong Province - Department of Science and Technology of Shandong Province[2018GHY115031] ; Key R&D Program of Guangdong Province - Department of Science and Technology of Guangdong Province[2019B020215001] ; China Agriculture Research System - Ministry of Agriculture and Rural Affairs of the People's Republic of China[CARS-47]
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology ; Genetics & Heredity
WOS类目	Ecology ; Evolutionary Biology ; Genetics & Heredity
WOS记录号	WOS:000660881400004
出版者	BMC
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176862
专题	实验海洋生物学重点实验室
通讯作者	Li, Xian
作者单位	1.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Semicond, Beijing 100083, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Yunong,Zhou, Li,Wu, Lele,et al. Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus)[J]. BMC ECOLOGY AND EVOLUTION,2021,21(1):12.
APA	Wang, Yunong.,Zhou, Li.,Wu, Lele.,Song, Changbin.,Ma, Xiaona.,...&Li, Jun.(2021).Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus).BMC ECOLOGY AND EVOLUTION,21(1),12.
MLA	Wang, Yunong,et al."Evolutionary ecology of the visual opsin gene sequence and its expression in turbot (Scophthalmus maximus)".BMC ECOLOGY AND EVOLUTION 21.1(2021):12.

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