The dinoflagellate Alexandrium catenella producing only carbamate toxins may account for the seafood poisonings in Qinhuangdao, China

doi:10.1016/j.hal.2021.101980

	The dinoflagellate Alexandrium catenella producing only carbamate toxins may account for the seafood poisonings in Qinhuangdao, China
	Yu, Ren-Cheng1,2,3,4 ; Zhang, Qing-Chun 1,2; Liu, Yang5 ; Chen, Zhen-Fan 6; Geng, Hui-Xia7 ; Dai, Li 1,3; Lin, Zhuo-Ru 1,3; Tang, Wen-Jiao 8; Kong, Fan-Zhou1,2 ; Yan, Tian 1,2; Zhou, Ming-Jiang 1
	2021-03-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	103 页码:11
通讯作者	Yu, Ren-Cheng(rcyu@qdio.ac.cn)
摘要	An outbreak of paralytic shellfish poisoning, recorded in April 2016 in Qinhuangdao China, was suspected to be caused by a toxic species in genus Alexandrium. Shortly after the poisoning outbreak, shellfish and netconcentrated phytoplankton samples were collected from the Bohai Sea, and analysed using high performance liquid chromatography coupled with fluorescence detection. Paralytic shellfish toxins (PSTs) were detected in both phytoplankton and shellfish samples, with similar toxin profiles dominated by carbamate toxins. High throughput sequencing data for phytoplankton samples collected previously in the coastal waters of Qinhuangdao were then analysed, and 8 operational taxonomic units (OTUs) were assigned to Alexandrium affine, A. andersonii/A. ostenfeldii, A. catenella, A. fraterculus, A. hiranoi/A. pseudogonyaulax, A. margalefii, A. pacificum and A. pohangense, among which A. catenella, A. pacificum and A. ostenfeldii could be potential producers of PSTs. During a cruise in 2019, three isolates of Alexandrium were established by cyst germination, and identified as A. catenella based on the sequences of the 28S ribosomal RNA gene (28S rDNA) D1-D2 region. Interestingly, all the three strains had the same toxin profile consisting of gonyautoxins 1, 3, 4 (GTX1, 3, 4) and neosaxitoxin (NEO). The toxin profile is similar to those of phytoplankton samples collected previously in the coastal waters of Qinhuangdao, but remarkably different from the general toxin profile of A. catenella dominated by N-sulfocarbamoyl toxins C1-2 in the Bohai Sea and the Yellow Sea. The results suggest that A. catenella is most likely to be the causative species of the poisoning outbreak in Qinhuangdao. As far as we know, this is the first report of A. catenella in the Bohai Sea producing PSTs dominated by high potent gonyautoxins GTX1-4. Occurrence of the highly toxic A. catenella will increase the risk of paralytic shellfish poisoning, which necessitates in-depth mechanism studies and increasing monitoring efforts.
关键词	Paralytic shellfish toxins (PSTs) Alexandrium catenella Bohai Sea HPLC High throughput sequencing Cyst
DOI	10.1016/j.hal.2021.101980
收录类别	SCI
语种	英语
资助项目	National Key RD Program[2019YFC1407901] ; Science & Technology Basic Resources Investigation Program[2018FY100206] ; Ministry of Science and Technology of China ; CAS-CSIRO BAU project[GJHZ201973] ; Chinese Academy of Sciences
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000663075400011
出版者	ELSEVIER
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176868
专题	海洋生态与环境科学重点实验室
通讯作者	Yu, Ren-Cheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Peoples R China 6.Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen 518060, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, Changjiang River Estuary Ecosyst Res Stn, Qingdao 266071, Peoples R China 8.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yu, Ren-Cheng,Zhang, Qing-Chun,Liu, Yang,et al. The dinoflagellate Alexandrium catenella producing only carbamate toxins may account for the seafood poisonings in Qinhuangdao, China[J]. HARMFUL ALGAE,2021,103:11.
APA	Yu, Ren-Cheng.,Zhang, Qing-Chun.,Liu, Yang.,Chen, Zhen-Fan.,Geng, Hui-Xia.,...&Zhou, Ming-Jiang.(2021).The dinoflagellate Alexandrium catenella producing only carbamate toxins may account for the seafood poisonings in Qinhuangdao, China.HARMFUL ALGAE,103,11.
MLA	Yu, Ren-Cheng,et al."The dinoflagellate Alexandrium catenella producing only carbamate toxins may account for the seafood poisonings in Qinhuangdao, China".HARMFUL ALGAE 103(2021):11.

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