Chronic nitrate exposure cause alteration of blood physiological parameters, redox status and apoptosis of juvenile turbot (Scophthalmus maximus)

doi:10.1016/j.envpol.2021.117103

IOCAS-IR > 实验海洋生物学重点实验室

	Chronic nitrate exposure cause alteration of blood physiological parameters, redox status and apoptosis of juvenile turbot (Scophthalmus maximus)
	Yu, Jiachen 1,2,3,4; Xiao, Yongshuang 1,2,3; Wang, Yanfeng 1,2,3; Xu, Shihong 1,2,3; Zhou, Li 1,2,3,4; Li, Jun 1,2,3; Li, Xian 5
	2021-08-15
发表期刊	ENVIRONMENTAL POLLUTION
ISSN	0269-7491
卷号	283 页码:11
通讯作者	Li, Jun(junli@qdio.ac.cn) ; Li, Xian(lixian@qdio.ac.cn)
摘要	Nitrate (NO3-) is one of the common inorganic nitrogen compound pollutants in natural ecosystems, which may have serious risks for aquatic organisms. However, its toxicological mechanism remains unclear. In the current study, juvenile turbot (Scophthalmus maximus) were exposed to different concentrations of NO3- (CK- 3.57 +/- 0.16, LN - 60.80 +/- 1.21, MN - 203.13 +/- 10.97 and HN - 414.16 +/- 15.22 mg/L NO3-N) for 60 d. The blood biochemical assays results revealed that elevated NO3- exposure significantly increased the concentrations of plasma NO3-, NO2-, MetHb, K+, cortisol, glucose, triglyceride, lactate, while significantly decreased the concentrations of plasma Hb, Na+ and Cl-, which meant that NO3- caused hypoxic stress and further affected the osmoregulation and metabolism in fish. Besides, exposure to MN and HN induced a significant decrease in the level of antioxidants, including SOD (Point: 60th day, MN, HN v.s. CK: 258.36, 203.73 v.s. 326.95 U/mL), CAT (1.97, 1.17 v.s. 2.37 U/mL), GSH (25.38, 20.74 v.s. 37.00 mmol/L), and GPx (85.32, 71.46 v.s. 129.36 U/mL), and a significant increase of MDA (7.54, 9.73 v.s. 5.27 nmol/L), suggesting that NO3- exposure leading to a disruption of the redox status in fish. Also, further research revealed that NO3- exposure altered the mRNA levels of p53 (HN: up to 4.28 folds) and p53-regulated downstream genes such as Bcl-2 (inferior to 0.44 folds), caspase-3 (up to 2.90 folds) and caspase-7 (up to 3.49 folds), indicating that NO3- exposure induced abnormal apoptosis in the fish gills. Moreover, IBRv2 analysis showed that the toxicity of NO3- exposure to turbot was dose-dependent, and the toxicity peaked on the 15th day. In short, NO3- is an environmental toxicological factor that cannot be ignored, because its toxic effects are long-term and could cause irreversible damage to fish. These results would be beneficial to improve our understanding of the toxicity mechanism of NO3- to fish, which provides baseline evidence for the risk assessment of environmental NO3- in aquatic ecosystems. (C) 2021 Elsevier Ltd. All rights reserved.
关键词	Nitrate exposure Blood physiological parameters Redox status Apoptosis Scophthalmus maximus
DOI	10.1016/j.envpol.2021.117103
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program[2018YFD0901204] ; China Agriculture Research System[CARS47G21] ; STS project[KFZDSW106] ; STS project[ZSSD019] ; STS project[2017T3017] ; STS project[KFJSTSQYZX020] ; Qingdao National Laboratory for Marine Science and Technology[2018SDKJ05022] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Natural Science Foundation of China[31672672]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000663133200010
出版者	ELSEVIER SCI LTD
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176883
专题	实验海洋生物学重点实验室
通讯作者	Li, Jun; Li, Xian
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yu, Jiachen,Xiao, Yongshuang,Wang, Yanfeng,et al. Chronic nitrate exposure cause alteration of blood physiological parameters, redox status and apoptosis of juvenile turbot (Scophthalmus maximus)[J]. ENVIRONMENTAL POLLUTION,2021,283:11.
APA	Yu, Jiachen.,Xiao, Yongshuang.,Wang, Yanfeng.,Xu, Shihong.,Zhou, Li.,...&Li, Xian.(2021).Chronic nitrate exposure cause alteration of blood physiological parameters, redox status and apoptosis of juvenile turbot (Scophthalmus maximus).ENVIRONMENTAL POLLUTION,283,11.
MLA	Yu, Jiachen,et al."Chronic nitrate exposure cause alteration of blood physiological parameters, redox status and apoptosis of juvenile turbot (Scophthalmus maximus)".ENVIRONMENTAL POLLUTION 283(2021):11.

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