A genome-wide association study to identify the genes associated with shell growth and shape-related traits in Crassostrea gigas

doi:10.1016/j.aquaculture.2021.736926

IOCAS-IR > 实验海洋生物学重点实验室

	A genome-wide association study to identify the genes associated with shell growth and shape-related traits in Crassostrea gigas
	He, Xin 1,2,3,4,7; Li, Chunyan1,2,3 ; Qi, Haigang 1,2,3,6; Meng, Jie1,2,3,6 ; Wang, Wei 1,2,3,6; Wu, Fucun 1,2,3,6,7; Li, Li 1,2,5,6; Zhang, Guofan 1,2,3,6
	2021-10-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	543 页码:9
通讯作者	Wu, Fucun(wufucun@qdio.ac.cn)
摘要	Oyster shell traits are economically important for commercial trade, and can directly reflect the quality of oysters, and thus affect consumer purchasing decisions. However, few studies have been conducted on the molecular mechanisms of oyster shell growth and shaping. For the Pacific oyster, Crassostrea gigas, we measured shell related traits of F1 individuals from 427 half-sib families at four growth stages. We defined shell height (SH), shell length (SL), and shell width (SW) as shell growth-related traits, while secondary traits of shell length to shell height ratio (SLSH) and shell width-to-shell height ratio (SWSH) were defined as shell shape-related traits. Further, we identified the genes associated with these traits using genome-wide association studies (GWAS). A total of 212 candidate single nucleotide polymorphism (SNP) markers were found to be associated with related traits. Then, 66 candidate genes that may relate to oyster shell growth within 100 kb upstream and downstream of these SNPs were selected for further study. We validated these genes in the extreme phenotypic groups of another independent population. Finally, we obtained seven genes (Rpsa, Trim3, Tnn, Ky, Fndc2, SLC7A9, and Ankrd44) with significantly different expressions in these groups, six of which were associated with secondary shell shape-related traits. Our results fill a research gap in oyster shell shaping and may serve as a reference for future selective breeding.
关键词	Crassostrea gigas Shell growth Shell shape GWAS
DOI	10.1016/j.aquaculture.2021.736926
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY010813] ; National Key R&D Program of China[2019YFD0900800] ; China Agriculture Research System of MOF ; China Agriculture Research System of MARA
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000672589700003
出版者	ELSEVIER
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176982
专题	实验海洋生物学重点实验室
通讯作者	Wu, Fucun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 5.Pilot Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 6.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	He, Xin,Li, Chunyan,Qi, Haigang,et al. A genome-wide association study to identify the genes associated with shell growth and shape-related traits in Crassostrea gigas[J]. AQUACULTURE,2021,543:9.
APA	He, Xin.,Li, Chunyan.,Qi, Haigang.,Meng, Jie.,Wang, Wei.,...&Zhang, Guofan.(2021).A genome-wide association study to identify the genes associated with shell growth and shape-related traits in Crassostrea gigas.AQUACULTURE,543,9.
MLA	He, Xin,et al."A genome-wide association study to identify the genes associated with shell growth and shape-related traits in Crassostrea gigas".AQUACULTURE 543(2021):9.

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