RNA-Seq analysis and WGCNA reveal dynamic molecular responses to air exposure in the hard clam Mercenaria mercenaria

doi:10.1016/j.ygeno.2021.06.025

	RNA-Seq analysis and WGCNA reveal dynamic molecular responses to air exposure in the hard clam Mercenaria mercenaria
	Zhou, Cong1,2,4,5,6 ; Song, Hao 1,2,3,4,6; Feng, Jie 1,2,4,6; Hu, Zhi 1,2,4,5,6; Yu, Zheng-Lin 1,2,4,5,6; Yang, Mei-Jie 1,2,4,5,6; Shi, Pu1,2,4,5,6 ; Li, Yong-Ren 7; Guo, Yong-Jun 7; Zhang, Tao1,2,3,4,6
	2021-07-01
发表期刊	GENOMICS
ISSN	0888-7543
卷号	113 期号:4 页码:2847-2859
通讯作者	Zhang, Tao(tzhang@qdio.ac.cn)
摘要	Intertidal bivalves are constantly exposed to air due to daily and seasonal tidal cycles. The hard clam Mercenaria mercenaria is an economically important bivalve species and often subjected to air exposure for more than 10 days during long-distance transportation. Hard clam exhibits remarkable tolerance to air exposure. In this study, we performed RNA sequencing on hemocytes of M. mercenaria exposed to air for 0, 1, 5, 10, 20 and 30 days. The overall and dynamic molecular responses of hard clams to air exposure were revealed by different transcriptomic analysis strategies. As a result, most cytochrome P450 1A and 3A, and monocarboxylate transporter family members were up-regulated during air exposure. Additionally, the dominant molecular process in response to 5-d, 10-d, 20-d and 30-d air exposure was refolding of misfolded proteins in endoplasmic reticulum, lysosome-mediated degradation of phospholipids, protein metabolism and reorganization of cytoskeleton, and activation of anti-apoptotic process, respectively. Our results facilitated comprehensive understanding of the tolerance mechanisms of intertidal bivalves to air exposure.
关键词	Air exposure Mercenaria mercenaria Response Transcriptome
DOI	10.1016/j.ygeno.2021.06.025
收录类别	SCI
语种	英语
资助项目	earmarked fund for the Modern Agro-industry Technology Research System[CARS-49] ; Major Applied Technology Innovation Project in Agriculture of Shandong Province[SF1405303301] ; Industry Leading Talents Project of Taishan Scholars[LJNY201704] ; 'Double Hundred' Blue Industry Leader Team of Yantai ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[LMEESCTSP-2018-1] ; Science and Technology Project of Tianjin
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000669212300003
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177009
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aqua Ecol & Aquaculture, Tianjin 300384, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Cong,Song, Hao,Feng, Jie,et al. RNA-Seq analysis and WGCNA reveal dynamic molecular responses to air exposure in the hard clam Mercenaria mercenaria[J]. GENOMICS,2021,113(4):2847-2859.
APA	Zhou, Cong.,Song, Hao.,Feng, Jie.,Hu, Zhi.,Yu, Zheng-Lin.,...&Zhang, Tao.(2021).RNA-Seq analysis and WGCNA reveal dynamic molecular responses to air exposure in the hard clam Mercenaria mercenaria.GENOMICS,113(4),2847-2859.
MLA	Zhou, Cong,et al."RNA-Seq analysis and WGCNA reveal dynamic molecular responses to air exposure in the hard clam Mercenaria mercenaria".GENOMICS 113.4(2021):2847-2859.

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