Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei

doi:10.1016/j.aquaculture.2021.736922

IOCAS-IR > 实验海洋生物学重点实验室

	Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei
	Wang, Yue 1,2,3; Yu, Yang1,2,4 ; Li, Shihao 1,2,4; Zhang, Xiaojun 1,2,4; Li, Fuhua 1,2,4,5
	2021-10-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	543 页码:6
通讯作者	Yu, Yang(yuyang@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
摘要	In order to understand the characteristics of the sex determination region in Litopenaeus vannamei, genome walking was performed based on the existing sex-linked Marker19299. A fragment of 2630 bp sequence was obtained for the genomic DNA of females, which contains two contigs, contig 122401 and contig 4497889. On contig 122401, the sequence showed a sex-biased amplification between females and males, the target band for males was much weaker than that for females. Copy number analysis showed that the female DNA contained more copies of contig 122401 sequence than that of males. Besides, the females contained an extra 9 bp insertion and a 205 bp repeat on contig 4497889 in comparison with males. These results not only provide further evidence for the ZZ/ZW sex determination system in Litopenaeus vannamei, but also suggest a novel hypothesis that the sex determination in L.vannamei might be controlled by the existence of the differences both in DNA sequence and genome structure. According to the above characteristics, a fast detection method to distinguish genetic sex of L.vannamei was developed. This research will not only provide useful information for the fine mapping of the sex determination region in L.vannamei, but also accelerate studies about the sex determination mechanism in shrimp.
关键词	Sex determination region Amplification difference Sequence difference Genetic sex detection Litopenaeus vannamei
DOI	10.1016/j.aquaculture.2021.736922
收录类别	SCI
语种	英语
资助项目	Joint NSFC-ISF Research Program[31861143047] ; National Key R&D Program of China[2018YFD0900202] ; China Agriculture Research System-48 (CARS-48)
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000672589700012
出版者	ELSEVIER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177021
专题	实验海洋生物学重点实验室
通讯作者	Yu, Yang; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, Yue,Yu, Yang,Li, Shihao,et al. Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei[J]. AQUACULTURE,2021,543:6.
APA	Wang, Yue,Yu, Yang,Li, Shihao,Zhang, Xiaojun,&Li, Fuhua.(2021).Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei.AQUACULTURE,543,6.
MLA	Wang, Yue,et al."Analysis of a sex-biased sequence in sex determination region and exploitation of a fast sex detection method in Litopenaeus vannamei".AQUACULTURE 543(2021):6.

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