Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency

doi:10.1016/j.biortech.2021.126484

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	Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency
	Yu, Wenjie 1,2; Zhang, Litao 1,3; Zhao, Jing 1; Liu, Jianguo 1,3,4
	2022-02-01
发表期刊	BIORESOURCE TECHNOLOGY
ISSN	0960-8524
卷号	345 页码:10
通讯作者	Liu, Jianguo(jgliu@qdio.ac.cn)
摘要	Effective inducers and stress conditions play an essential role in the regulation of astaxanthin biosynthesis. This study reports a strategy developed by combining exogenous addition of oxaloacetate (OA) with nitrogen deficiency to facilitate astaxanthin production in Haematococcus pluvialis. Significantly, addition of 10 mM-OA enhanced the cellular astaxanthin content about 7.18-fold under nitrogen deficiency on day 7, with the content of astaxanthin esters increased concomitantly. To further elucidate the role and mechanism of OA on astaxanthin synthesis, the physiological and metabolic analyses of H. pluvialis treated with exogenous OA were performed. The results showed that exogenous OA promoted respiration over photosynthesis. Concurrently, the metabolite levels in the Embden-Meyerhof-Parnas pathway, pentose phosphate pathway and tricarboxylic acid cycle obviously increased. The enhancement of respiratory metabolic pathways led to elevated levels of substrates, thus directly promoted astaxanthin synthesis. The present findings provide a new and effective approach for optimizing astaxanthin production.
关键词	Oxaloacetate Astaxanthin accumulation Haematococcus pluvialis Nitrogen deficiency Respiratory metabolism
DOI	10.1016/j.biortech.2021.126484
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31872588] ; National Natural Science Foundation of China[U1706209] ; TaiShan Industrial Experts Programme[tscy20200102]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000732933200013
出版者	ELSEVIER SCI LTD
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177555
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Qingdao Langyatai Grp Co Ltd, Shandong Engn Technol Collaborat Innovat Ctr Edib, Qingdao 266400, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yu, Wenjie,Zhang, Litao,Zhao, Jing,et al. Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency[J]. BIORESOURCE TECHNOLOGY,2022,345:10.
APA	Yu, Wenjie,Zhang, Litao,Zhao, Jing,&Liu, Jianguo.(2022).Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency.BIORESOURCE TECHNOLOGY,345,10.
MLA	Yu, Wenjie,et al."Enhancement of astaxanthin accumulation in Haematococcus pluvialis by exogenous oxaloacetate combined with nitrogen deficiency".BIORESOURCE TECHNOLOGY 345(2022):10.

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