Construction of a High-Density Genetic Linkage Map for the Mapping of QTL Associated with Growth-Related Traits in Sea Cucumber (Apostichopus japonicus)

doi:10.3390/biology11010050

	Construction of a High-Density Genetic Linkage Map for the Mapping of QTL Associated with Growth-Related Traits in Sea Cucumber (Apostichopus japonicus)
	Cui, Wei1,2,3,4,5,6 ; Huo, Da1,2,3,4,6 ; Liu, Shilin 1,2,3,4,6; Xing, Lili1,2,3,4,5,6 ; Su, Fang 1,2,3,4,5,6; Yang, Hongsheng1,2,3,4,5,6,7 ; Sun, Lina 1,2,3,4,5,6
	2022
发表期刊	BIOLOGY-BASEL
卷号	11 期号:1 页码:16
通讯作者	Sun, Lina(sunlina@qdio.ac.cn)
摘要	Simple Summary Slow growth and germplasm degradation have restricted the sustainable commercial development of the sea cucumber industry. To analyze the genetic mechanism of growth traits of sea cucumbers, we constructed a high-density genetic linkage map based on single nucleotide polymorphism (SNP) molecular markers and performed a quantitative trait loci (QTL) mapping analysis. We annotated a critical candidate gene related to growth traits and explored mRNA expression levels. The results showed that the gene was significantly highly expressed during the larval developmental stages. These results can be used to genetically improve the growth traits of sea cucumbers. Genetic linkage maps have become an indispensable tool for genetics and genomics research. Sea cucumber (Apostichopus japonicus), which is an economically important mariculture species in Asia, is an edible echinoderm with medicinal properties. In this study, the first SNP-based high-density genetic linkage map was constructed by sequencing 132 A. japonicus individuals (2 parents and 130 offspring) according to a genotyping-by-sequencing (GBS) method. The consensus map was 3181.54 cM long, with an average genetic distance of 0.52 cM. A total of 6144 SNPs were assigned to 22 linkage groups (LGs). A Pearson analysis and QTL mapping revealed the correlations among body weight, body length, and papillae number. An important growth-related candidate gene, protein still life, isoforms C/SIF type 2 (sif), was identified in LG18. The gene was significantly highly expressed during the larval developmental stages. Its encoded protein reportedly functions as a guanine nucleotide exchange factor. These results would facilitate the genetic analysis of growth traits and provide valuable genomic resources for the selection and breeding of new varieties of sea cucumbers with excellent production traits.
关键词	GBS growth traits genetic linkage map SNP QTL marker-assisted selection breeding sea cucumber (Apostichopus japonicus)
DOI	10.3390/biology11010050
收录类别	SCI
语种	英语
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:000750573300001
出版者	MDPI
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177976
专题	海洋生态与环境科学重点实验室
通讯作者	Sun, Lina
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Cui, Wei,Huo, Da,Liu, Shilin,et al. Construction of a High-Density Genetic Linkage Map for the Mapping of QTL Associated with Growth-Related Traits in Sea Cucumber (Apostichopus japonicus)[J]. BIOLOGY-BASEL,2022,11(1):16.
APA	Cui, Wei.,Huo, Da.,Liu, Shilin.,Xing, Lili.,Su, Fang.,...&Sun, Lina.(2022).Construction of a High-Density Genetic Linkage Map for the Mapping of QTL Associated with Growth-Related Traits in Sea Cucumber (Apostichopus japonicus).BIOLOGY-BASEL,11(1),16.
MLA	Cui, Wei,et al."Construction of a High-Density Genetic Linkage Map for the Mapping of QTL Associated with Growth-Related Traits in Sea Cucumber (Apostichopus japonicus)".BIOLOGY-BASEL 11.1(2022):16.

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