Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
Zeolitic Imidazolate Framework-8 Grown on Nanoporous SiO2 with Aptamer Functionalization for Selective Isolation of Thrombin | |
Lin, Yanna1; Xu, Yulong1; Xing, Yanzhi1; Liu, Nazhen2; Chen, Xuwei1 | |
2021-11-26 | |
发表期刊 | ACS APPLIED NANO MATERIALS |
卷号 | 4期号:11页码:12204-12212 |
通讯作者 | Liu, Nazhen(liunazhen@qdio.ac.cn) ; Chen, Xuwei(chenxuwei@mail.neu.edu.cr) |
摘要 | A three-dimensional ordered macroporous composite (3DOM SiO2@ZIF-8-TBA) was fabricated via the in situ growth of metal-organic framework ZIF-8 (similar to 50 nm) on the surface of macroporous SiO2 (pore size of similar to 250 nm) and the subsequent functionalization with a thrombin aptamer. The obtained 3DOM SiO2@ZIF-8-TBA composite has been proven to be a potential protein-enriching adsorbent, which exhibits high selectivity toward the adsorption of thrombin due to the specific conformation between the aptamer and thrombin. The adsorption behavior of thrombin onto the 3DOM SiO2@ZIF-8-TBA composite conforms to the Langmuir adsorption model, resulting in a superior adsorption capacity of 40.9 U mg(-1), contributed by the ordered macroporous structure. The retained thrombin could be readily recovered by 1.0% NH3 center dot H2O (m/v) with a recovery of 92.3%. Circular dichroism spectra illustrate that the 3DOM SiO2@ZIF-8-TBA composite-based adsorption/desorption processes have no influence on thrombin conformation. The application of the 3DOM SiO2@ZIF-8-TBA composite in separating thrombin from human serum has been verified; fibrin production tests prove that the activity of recovered thrombin is well retained. |
关键词 | 3D-ordered macroporous structure zeolitic imidazolate framework-8 nanoporous SiO2 solid-phase extraction aptamer thrombin |
DOI | 10.1021/acsanm.1c02691 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Natural Science Foundation of China[21727811] ; Fundamental Research Funds for the Central Universities[N2005027] |
WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
WOS类目 | Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
WOS记录号 | WOS:000752894100083 |
出版者 | AMER CHEMICAL SOC |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/178006 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Liu, Nazhen; Chen, Xuwei |
作者单位 | 1.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Shenyang 110819, Peoples R China 2.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, Qingdao 266071, Peoples R China |
通讯作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Lin, Yanna,Xu, Yulong,Xing, Yanzhi,et al. Zeolitic Imidazolate Framework-8 Grown on Nanoporous SiO2 with Aptamer Functionalization for Selective Isolation of Thrombin[J]. ACS APPLIED NANO MATERIALS,2021,4(11):12204-12212. |
APA | Lin, Yanna,Xu, Yulong,Xing, Yanzhi,Liu, Nazhen,&Chen, Xuwei.(2021).Zeolitic Imidazolate Framework-8 Grown on Nanoporous SiO2 with Aptamer Functionalization for Selective Isolation of Thrombin.ACS APPLIED NANO MATERIALS,4(11),12204-12212. |
MLA | Lin, Yanna,et al."Zeolitic Imidazolate Framework-8 Grown on Nanoporous SiO2 with Aptamer Functionalization for Selective Isolation of Thrombin".ACS APPLIED NANO MATERIALS 4.11(2021):12204-12212. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论