Mechanisms of heat and hypoxia defense in hard clam: Insights from transcriptome analysis

doi:10.1016/j.aquaculture.2021.737792

	Mechanisms of heat and hypoxia defense in hard clam: Insights from transcriptome analysis
	Hu, Zhi 1,2,3,4,5,6; Feng, Jie 1,2,3,4,6; Song, Hao 1,2,3,4,6; Zhou, Cong1,2,3,4,5,6 ; Yu, Zheng-Lin 1,2,3,4,6; Yang, Mei-Jie 1,2,3,4,5,6; Shi, Pu1,2,3,4,5,6 ; Guo, Yong-Jun 7; Li, Yong-Ren 7; Zhang, Tao1,2,3,4,6
	2022-02-25
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	549 页码:11
通讯作者	Zhang, Tao(tzhang@qdio.ac.cn)
摘要	With global changes and anthropogenic activities, heat and hypoxia have become frequent stressors in aquatic environments. However, the molecular defense mechanisms of aquatic organisms, especially bivalves, against the synergistic effects of heat and hypoxia have not been fully elucidated. In this study, we analyzed transcriptomic data of the gills of Mercenaria mercenaria, an economically and ecologically important bivalve, under several stress challenges: high temperature (heat), low dissolved oxygen (hypoxia), and heat plus hypoxia. GO and KEGG enrichment analyses were performed on the differentially expressed gene sets of heat-induced genes under various dissolved oxygen (DO) solubilities, hypoxia-induced genes under different (normal and high) temperatures and common heat plus hypoxia stress-induced genes. We found that protein-folding item was significantly enriched in common differentially expressed heat-induced genes comparisons, with a high expression of molecular chaperones HSP90 and TCP1. Moreover, microtubule-related GO terms were significantly enriched in differentially expressed hypoxia-induced genes under different temperatures, and kinesin and dynein-related genes were commonly upregulated in the hypoxia challenge groups. Protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis were the most significantly enriched pathways in common heat plus hypoxia stress-induced genes. Furthermore, correctly-folding related genes, luminal chaperones, and ER associated degradation related genes were upregulated under heat plus hypoxia stresses. Altogether, our results provided new insights on understanding the molecular defense mechanisms against heat and hypoxia stress in hard clams.
关键词	Mercenaria mercenaria RNA-seq Heat Hypoxia Global change
DOI	10.1016/j.aquaculture.2021.737792
收录类别	SCI
语种	英语
资助项目	China National Key RD Program[2019YFD0900800] ; Tianjin Science and Technology Commission Project[20YFZCSN00240] ; Tianjin Agricultural Committee Project[202103010] ; China Agriculture Research System of MOF ; Shandong Province Agricultural Major Applied Technology Innovation Project[SF1405303301] ; Taishan Industrial Leading Talents Project[LJNY201704] ; 'Double Hundred' Blue Industry Leader Team of Yantai ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1] ; China Agriculture Research System of MARA
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000765399200007
出版者	ELSEVIER
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178456
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Tianjin Agr Univ, Fisheries Coll, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin 300384, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Zhi,Feng, Jie,Song, Hao,et al. Mechanisms of heat and hypoxia defense in hard clam: Insights from transcriptome analysis[J]. AQUACULTURE,2022,549:11.
APA	Hu, Zhi.,Feng, Jie.,Song, Hao.,Zhou, Cong.,Yu, Zheng-Lin.,...&Zhang, Tao.(2022).Mechanisms of heat and hypoxia defense in hard clam: Insights from transcriptome analysis.AQUACULTURE,549,11.
MLA	Hu, Zhi,et al."Mechanisms of heat and hypoxia defense in hard clam: Insights from transcriptome analysis".AQUACULTURE 549(2022):11.

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