Diatom Biodiversity and Speciation Revealed by Comparative Analysis of Mitochondrial Genomes

doi:10.3389/fpls.2022.749982

	Diatom Biodiversity and Speciation Revealed by Comparative Analysis of Mitochondrial Genomes
	Wang, Yichao1,2,3,4 ; Liu, Shuya 1,2,4; Wang, Jing1,2,4 ; Yao, Yanxin1,2,3,4 ; Chen, Yang1,2,3,4 ; Xu, Qing 1,2,4,5; Zhao, Zengxia 2,6; Chen, Nansheng 1,2,4,7
	2022-03-24
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	13 页码:20
通讯作者	Chen, Nansheng(chenn@qdio.ac.cn)
摘要	Diatoms (Bacillariophyta) constitute one of the most diverse and ecologically significant groups of phytoplankton, comprising 100,000-200,000 species in three classes Bacillariophyceae, Mediophyceae, and Coscinodiscophyceae. However, due to the limited resolution of common molecular markers including 18S rDNA, 28S rDNA, ITS, rbcL, and cox1, diatom biodiversity has not been adequately ascertained. Organelle genomes including mitochondrial genomes (mtDNAs) have been proposed to be "super barcodes" for distinguishing diatom species because of their rich genomic content, and the rapid progress of DNA sequencing technologies that has made it possible to construct mtDNAs with increasing throughout and decreasing cost. Here, we constructed complete mtDNAs of 15 diatom species including five Coscinodiscophyceae species (Guinardia delicatula, Guinardia striata, Stephanopyxis turris, Paralia sulcata, and Actinocyclus sp.), four Mediophyceae species (Hemiaulus sinensis, Odontella aurita var. minima, Lithodesmioides sp., and Helicotheca tamesis), and six Bacillariophyceae species (Nitzschia ovalis, Nitzschia sp., Nitzschia traheaformis, Cylindrotheca closterium, Haslea tsukamotoi, and Pleurosigma sp.) to test the practicality of using mtDNAs as super barcodes. We found that mtDNAs have much higher resolution compared to common molecular markers as expected. Comparative analysis of mtDNAs also suggested that mtDNAs are valuable in evolutionary studies by revealing extensive genome rearrangement events with gene duplications, gene losses, and gains and losses of introns. Synteny analyses of mtDNAs uncovered high conservation among species within an order, but extensive rearrangements including translocations and/or inversions between species of different orders within a class. Duplication of cox1 was discovered for the first time in diatoms in Nitzschia traheaformis and Haslea tsukamotoi. Molecular dating analysis revealed that the three diatom classes split 100 Mya and many diatom species appeared since 50 Mya. In conclusion, more diatom mtDNAs representing different orders will play great dividends to explore biodiversity and speciation of diatoms in different ecological regions.
关键词	diatom mitochondrial genome comparative genomics evolution diversity divergence time
DOI	10.3389/fpls.2022.749982
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan[19-3-2-16-zhc] ; National Key Research and Development Program of China[2017YFC1404300]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000781975200001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/178832
专题	海洋生态与环境科学重点实验室胶州湾海洋生态系统国家野外研究站
通讯作者	Chen, Nansheng
作者单位	1.Chinese Acad Sci, Chinese Acad Sci Key Lab Marine Ecol & Environm S, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Nat Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Planetary & Earth Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Natl Marine Ecosyst Res Stn, Qingdao, Peoples R China 7.Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC, Canada
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Yichao,Liu, Shuya,Wang, Jing,et al. Diatom Biodiversity and Speciation Revealed by Comparative Analysis of Mitochondrial Genomes[J]. FRONTIERS IN PLANT SCIENCE,2022,13:20.
APA	Wang, Yichao.,Liu, Shuya.,Wang, Jing.,Yao, Yanxin.,Chen, Yang.,...&Chen, Nansheng.(2022).Diatom Biodiversity and Speciation Revealed by Comparative Analysis of Mitochondrial Genomes.FRONTIERS IN PLANT SCIENCE,13,20.
MLA	Wang, Yichao,et al."Diatom Biodiversity and Speciation Revealed by Comparative Analysis of Mitochondrial Genomes".FRONTIERS IN PLANT SCIENCE 13(2022):20.

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