Nanostructure and Nanomechanics of Prorocentrum donghaiense and Their Changes Under Nitrogen Limitation by Atomic Force Microscopy

doi:10.3389/fmars.2022.874888

	Nanostructure and Nanomechanics of Prorocentrum donghaiense and Their Changes Under Nitrogen Limitation by Atomic Force Microscopy
	He, Liyan 1,2,3; Yu, Zhiming1,2,3,4 ; Zhu, Jianan 1,2,3; Cao, Xihua1,2,3 ; Song, Xiuxian1,2,3,4
	2022-04-28
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	9 页码:12
通讯作者	Yu, Zhiming(zyu@qdio.ac.cn)
摘要	In this paper, atomic force microscopy was employed to study the harmful algal bloom species Prorocentrum donghaiense for the first time. Cells were immobilized in pores of polycarbonate membrane to keep moist and to acquire images of P. donghaiense at different scales. Typical ultrastructures, such as knob-like spines and valve pores, were observed on cell surfaces. These structures had similar characteristics to those observed on scanning electron microscopy images. Moreover, the height and spacing of typical nanostructure, and nanomechanical parameters such as adhesion and elasticity, were also quantified by AFM. Additionally, the changes in cell surface nanostructure and nanomechanical characteristics under nitrogen limitation were further studied. Compared with the cells under normal culture conditions, the cell surface roughness and adhesion decreased, and the elastic modulus increased for cells under nitrogen limitation. Potential changes in the ability of P. donghaiense cells to perform normal physiological functions are reflected by changes in cell surface parameters, including cell surface roughness, cell surface adhesion, and cell surface elasticity. The results of this study reveal how P. donghaiense responds to changes in the external environment under approximately physiological conditions from the perspective of changes in cell surface nanostructures and nanomechanical characteristics and provide a new understanding of its cell biology.
关键词	Prorocentrum donghaiense atomic force microscopy nanostructure nanomechanics nitrogen limitation
DOI	10.3389/fmars.2022.874888
收录类别	SCI
语种	英语
资助项目	Taishan Program of Shandong Province ; Youth Talent Support Program of the Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao)[LMEES-YTSP-2018-01-03] ; National Natural Science Foundation of China[42006120]
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000795886000001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179201
专题	海洋生态与环境科学重点实验室
通讯作者	Yu, Zhiming
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	He, Liyan,Yu, Zhiming,Zhu, Jianan,et al. Nanostructure and Nanomechanics of Prorocentrum donghaiense and Their Changes Under Nitrogen Limitation by Atomic Force Microscopy[J]. FRONTIERS IN MARINE SCIENCE,2022,9:12.
APA	He, Liyan,Yu, Zhiming,Zhu, Jianan,Cao, Xihua,&Song, Xiuxian.(2022).Nanostructure and Nanomechanics of Prorocentrum donghaiense and Their Changes Under Nitrogen Limitation by Atomic Force Microscopy.FRONTIERS IN MARINE SCIENCE,9,12.
MLA	He, Liyan,et al."Nanostructure and Nanomechanics of Prorocentrum donghaiense and Their Changes Under Nitrogen Limitation by Atomic Force Microscopy".FRONTIERS IN MARINE SCIENCE 9(2022):12.

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