Integrative omics analysis highlights the immunomodulatory effects of the parasitic dinoflagellate hhematodinium on crustacean hemocytes

doi:10.1016/j.fsi.2022.04.050

	Integrative omics analysis highlights the immunomodulatory effects of the parasitic dinoflagellate hhematodinium on crustacean hemocytes
	Li, Meng1,2,3 ; Huang, Qian 1,2,4; Lv, Xiaoyang 1,2; Small, Hamish J.5; Li, Caiwen 1,2,3,4
	2022-06-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	125 页码:35-47
通讯作者	Small, Hamish J.(hamish@vims.edu) ; Li, Caiwen(cwli@qdio.ac.cn)
摘要	Parasitic dinoflagellates in genus Hematodinium have caused substantial economic losses to multiple commercially valuable marine crustaceans around the world. Recent efforts to better understand the life cycle and biology of the parasite have improved our understanding of the disease ecology. However, studies on the hostparasite interaction, especially how Hematodinium parasites evade the host immune response are lacking. To address this shortfall, we used the comprehensive omics approaches (miRNA transcriptomics, iTRAQ-based proteomics) to get insights into the host-parasite interaction between hemocytes from Portunus trituberculatus and Hematodinium perezi in the present study. The parasitic dinoflagellate H. perezi remodeled the miRNome and proteome of hemocytes from challenged hosts, modulated the host immune response at both post-transcriptional and translational levels and caused post-transcriptional regulation to the host immune response. Multiple important cellular and humoral immune-related pathways (ex. Apoptosis, Endocytosis, ECM-receptor interaction, proPO activation pathway, Toll-like signaling pathway, Jak-STAT signaling pathway) were significantly affected by Hematodinium parasites. Through modulation of the host miRNome, the host immune responses of nodulation, proPO activation and antimicrobial peptides were significantly suppressed. Cellular homeostasis was imbalanced via post-transcriptional dysregulation of the phagosome and peroxisome pathways. Cellular structure and communication was seriously impacted by post-transcriptional downregulation of ECM-receptor interaction and focal adhesion pathways. In conclusion, H. perezi parasites could trigger striking changes in the miRNome and proteome of crustacean hemocytes, and this parasite exhibited multifaceted immunomodulatory effects and potential immune-suppressive mechanisms in crustacean hosts.
关键词	Parasitic dinoflagellates Hematodinium Crustacean Innate immunity Host-parasite interaction Omics study
DOI	10.1016/j.fsi.2022.04.050
收录类别	SCI
语种	英语
资助项目	NSFC-Shandong Joint-funding Program[U1906214] ; National Natural Science Foundation of China (NNSFC)[42006121] ; Key Deployment Project of Centre for Ocean Mega-Science, Chinese Academy of Sciences[COMS2020Q06] ; Youth Project of Natural Science Foundation of Shandong Province[ZR2020QD105] ; CPSF-CAS Joint Foundation for Excellent Postdoctoral Fellows[2016LH0034]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000803740900001
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179399
专题	海洋生态与环境科学重点实验室
通讯作者	Small, Hamish J.; Li, Caiwen
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Marine Ecol & Environm Sci Lab, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Virginia Inst Marine Sci, William & Mary,POB 1346, Gloucester Point, VA 23062 USA
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Li, Meng,Huang, Qian,Lv, Xiaoyang,et al. Integrative omics analysis highlights the immunomodulatory effects of the parasitic dinoflagellate hhematodinium on crustacean hemocytes[J]. FISH & SHELLFISH IMMUNOLOGY,2022,125:35-47.
APA	Li, Meng,Huang, Qian,Lv, Xiaoyang,Small, Hamish J.,&Li, Caiwen.(2022).Integrative omics analysis highlights the immunomodulatory effects of the parasitic dinoflagellate hhematodinium on crustacean hemocytes.FISH & SHELLFISH IMMUNOLOGY,125,35-47.
MLA	Li, Meng,et al."Integrative omics analysis highlights the immunomodulatory effects of the parasitic dinoflagellate hhematodinium on crustacean hemocytes".FISH & SHELLFISH IMMUNOLOGY 125(2022):35-47.

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