Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates

doi:10.1039/d2ay00416j

	Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates
	Ye, Xiangyi1,3 ; Zhang, Dun 1,2,3; Zeng, Yan1,3 ; Wang, Yingwen 1,2,3; Qi, Peng1,2,3
	2022-05-11
发表期刊	ANALYTICAL METHODS
ISSN	1759-9660
页码	7
通讯作者	Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn)
摘要	In this paper, we present a robust and versatile method for developing non-label aptasensors with high sensitivity. Amyloid-like protein aggregates were facilely synthesized with the commonly used passivating agent bovine serum albumin (BSA) in developing biosensors, and the produced amyloid-like phase-transited BSA (PTB) exhibited excellent antifouling performances and robust interfacial adhesion with the electrode surface. In order to improve the detection sensitivity of electrochemical measurements, reduced graphene oxide was electrochemically deposited onto the electrode surface. Moreover, gold nanoparticles were introduced to enhance the conductivity of the PTB film and facilitate subsequent aptamer modification. Two common biological species, adenosine triphosphate (ATP) and cytochrome c (cyt c), were chosen as detection targets, and their corresponding aptasensors were successfully constructed and systematically evaluated. The proposed aptasensors based on the PTB-Au antifouling composite exhibited high sensitivity and specificity towards ATP and cyt c detection, and the detection limits were calculated to be 0.26 nM and 0.64 nM for ATP and cyt c, respectively. Hence, this work provides a simple approach to develop highly sensitive aptasensors without any labeling process, and thus promises its great application in biological analysis.
DOI	10.1039/d2ay00416j
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[41906037] ; Nantong Scientific Plan Foundation[JC2021054] ; Nantong Scientific Plan Foundation[JC2020090]
WOS研究方向	Chemistry ; Food Science & Technology ; Spectroscopy
WOS类目	Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS记录号	WOS:000804237200001
出版者	ROYAL SOC CHEMISTRY
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179411
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Zhang, Dun; Qi, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Ye, Xiangyi,Zhang, Dun,Zeng, Yan,et al. Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates[J]. ANALYTICAL METHODS,2022:7.
APA	Ye, Xiangyi,Zhang, Dun,Zeng, Yan,Wang, Yingwen,&Qi, Peng.(2022).Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates.ANALYTICAL METHODS,7.
MLA	Ye, Xiangyi,et al."Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates".ANALYTICAL METHODS (2022):7.

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