Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection | |
Wei, Yangdao1; Tao, Zhenzhen1; Wan, Lu1; Zong, Chengli1; Wu, Jiajia2; Tan, Xiao3; Wang, Buhua1; Guo, Zixuan1; Zhang, Ling1; Yuan, Haoyu1; Wang, Peng2; Yang, Zhiqing1; Wan, Yi1 | |
2022-09-01 | |
发表期刊 | BIOSENSORS & BIOELECTRONICS |
ISSN | 0956-5663 |
卷号 | 211页码:6 |
通讯作者 | Yang, Zhiqing(yangzhiqing@hainanu.edu.cn) ; Wan, Yi(993602@hainanu.edu.cn) |
摘要 | CRISPR-Cas systems have been employed to detect a large variety of pathogenic microorganisms by simply changing the guide RNA sequence. However, these platforms usually rely on nucleic acid extraction and amplification to achieve good sensitivity. Herein, we developed a new platform for the highly specific and sensitive detection of live staphylococcus aureus (S. aureus) based on an Aptamer-based Cas14a1 Biosensor (ACasB), without the need for nucleic acid extraction or amplification. First, the S. aureus specific aptamer was hybrid with a blocker DNA. After the live S. aureus was added, the blocker can be released upon bacteria-aptamer binding. Finally, the released blocker can activate Cas14a1 protein by binding with the sgRNA to generate a change of fluorescent intensity. The ACasB indicates high specificity and sensitivity: it can directly distinguish 400 CFU/ml live S. aureus cells. Comparable to qPCR, the Cas14a1-aptamer biosensor can detect S. aureus with 100% accuracy in complex samples. Therefore, this ACasB for the on-site detection of live S. aureus can broaden its applications in food safety and environmental monitoring. |
关键词 | CRISPR-Cas Bacteria diagnostic Staphylococcus aureus |
DOI | 10.1016/j.bios.2022.114282 |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Hainan Provincial Natural Science Foundation of China, China[2019RC092] ; Hainan Provincial Natural Science Foundation of China, China[420RC518] ; Hainan Provincial Natural Science Foundation of China, China[821CXTD1011] ; National Natural Science Foundation of China, China[41866002] ; National Natural Science Foundation of China, China[4216060011] ; National Natural Science Foundation of China, China[82073697] ; Open Fund of Shandong Key Laboratory of Corrosion Science, China[KLCS201910] ; Research Foundation of Hainan University, China[KYQD (ZR) 1711] ; Research Foundation of Hainan University, China[KYQD (ZR) 1997] ; Research Foundation of Hainan University, China[KYQD (ZR) 20087] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology, China[ZD202003KF04] ; Open fund of Fujian Provincial Key Laboratory of Photonics Technology, China[JYG 2002] |
WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
WOS记录号 | WOS:000806344000003 |
出版者 | ELSEVIER ADVANCED TECHNOLOGY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/179438 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Yang, Zhiqing; Wan, Yi |
作者单位 | 1.Hainan Univ, Sch Pharmaceut Sci, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 3.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China |
推荐引用方式 GB/T 7714 | Wei, Yangdao,Tao, Zhenzhen,Wan, Lu,et al. Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection[J]. BIOSENSORS & BIOELECTRONICS,2022,211:6. |
APA | Wei, Yangdao.,Tao, Zhenzhen.,Wan, Lu.,Zong, Chengli.,Wu, Jiajia.,...&Wan, Yi.(2022).Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection.BIOSENSORS & BIOELECTRONICS,211,6. |
MLA | Wei, Yangdao,et al."Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection".BIOSENSORS & BIOELECTRONICS 211(2022):6. |
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