A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial

doi:10.3389/fimmu.2022.917697

IOCAS-IR

	A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial
	Li, Huili1,2,3,4,5 ; Sun, Yuanyuan1,2,3,4 ; Sun, Li 1,2,3,4,5
	2022-06-20
发表期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
卷号	13 页码:14
通讯作者	Sun, Li(lsun@qdio.ac.cn)
摘要	Chemokines are a group of cytokines that play important roles in cell migration, inflammation, and immune defense. In this study, we identified a CXC chemokine, CXCL10, from Japanese flounder Paralichthys olivaceus (named PoCXCL10) and investigated its immune function. Structurally, PoCXCL10 possesses an N-terminal coil, three beta-strands, and a C-terminal alpha-helix with cationic and amphipathic properties. PoCXCL10 expression occurred in multiple tissues and was upregulated by bacterial pathogens. Recombinant PoCXCL10 (rPoCXCL10) promoted the migration, cytokine expression, and phagocytosis of flounder peripheral blood leukocytes (PBLs). rPoCXCL10 bound to and inhibited the growth of a variety of common Gram-negative and Gram-positive fish pathogens. rPoCXCL10 killed the pathogens by causing bacterial membrane permeabilization and structure destruction. When introduced in vivo, rPoCXCL10 significantly inhibited bacterial dissemination in fish tissues. A peptide derived from the C-terminal alpha-helix exhibited bactericidal activity and competed with rPoCXCL10 for bacterial binding. Deletion of the alpha-helix affected the in vitro bactericidal activity but not the chemotaxis or in vivo antimicrobial activity of PoCXCL10. Together, these results indicate that PoCXCL10 exerts the role of both an immunoregulator and a bactericide/bacteriostatic via different structural domains. These findings provide new insights into the immune function and working mechanism of fish CXC chemokines.
关键词	Paralichthys olivaceus chemokine bacterial pathogen antimicrobial immune defense
DOI	10.3389/fimmu.2022.917697
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Immunology
WOS类目	Immunology
WOS记录号	WOS:000820146700001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179644
专题	中国科学院海洋研究所
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Qingdao, Peoples R China 2.Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Huili,Sun, Yuanyuan,Sun, Li. A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial[J]. FRONTIERS IN IMMUNOLOGY,2022,13:14.
APA	Li, Huili,Sun, Yuanyuan,&Sun, Li.(2022).A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial.FRONTIERS IN IMMUNOLOGY,13,14.
MLA	Li, Huili,et al."A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial".FRONTIERS IN IMMUNOLOGY 13(2022):14.