A calcification-related calmodulin-like protein in the oyster Crassostrea gigas mediates the enhanced calcium deposition induced by CO2 exposure

doi:10.1016/j.scitotenv.2022.155114

IOCAS-IR > 实验海洋生物学重点实验室

	A calcification-related calmodulin-like protein in the oyster Crassostrea gigas mediates the enhanced calcium deposition induced by CO2 exposure
	Wang, Xiudan1 ; Li, Changmei 1; Lv, Zhao 5; Zhang, Zhenqiang 1; Qiu, Limei 2,3,4
	2022-08-10
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	833 页码:11
通讯作者	Wang, Xiudan(wangxiudan@qust.edu.cn) ; Qiu, Limei(qiulimei@qdio.ac.cn)
摘要	Calcium transportation and homeostasis are essential for marine bivalves to maintain basic metabolism and build their shells. Calmodulin-like proteins (CaLPs) are important calcium sensors and buffers and can respond to ocean acidification (OA) in marine calcifiers. However, no further study of their physiological function in calcium metabolism under elevated CO2 has been performed. Here, we identified a novel CaLP (designated CgCaLP) in the Pacific oyster Crassostrea gigas and demonstrated its participation in the calcification process: the mRNA expression level of CgCaLP peaked at the trochophorc larval stage and remained high at stages when shells were shaped; the mRNA and protein of CgCaLP were more highly expressed in mantic tissue than in other tissues. Under elevated CO2 levels, the protein expression level of CgCaLP in hemocytes increased, while in contrast, significantly decreased protein levels were detected in gill and mantle tissues. Shell dissolution caused the imbalance of calcium in hemocytes and decreased calcium absorption and transportation demand in gill and mantle tissues, inducing the molecular function allocation of CgCaLP under CO2 exposure. Despite the decreased protein level in mantle tissue, CgCaLP was found to translocate to outer mantle epithelium (OME) cells where condensed calcium-rich deposits (CRDs) were detected. We further demonstrated that CgCaLP mRNA and protein expression levels could respond to seawater Ca2+ availability, suggesting that the calcium deposition capacity of oysters might be enhanced to fight against shell dissolution problems and that CgCaLP might serve as an essential participator of the process. In summary, CgCaLP might enhance calcium deposition under CO2 exposure and thus play a significant and flexible molecular function involved in a compensation strategy of oysters to fight against the acidified ocean.
关键词	Ocean acidification Crassostrea gigas CgCaLP Calcium deposition
DOI	10.1016/j.scitotenv.2022.155114
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Shandong Province[ZR2019BC040] ; National Natural Science Foundation of China[32072999] ; State Key Laboratory of Marine Environmental Science (Xiamen University)[MELRS2115]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000833001900006
出版者	ELSEVIER
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179879
专题	实验海洋生物学重点实验室
通讯作者	Wang, Xiudan; Qiu, Limei
作者单位	1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China 2.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 5.Hunan Agr Univ, Hunan Engn Technol Res Ctr Featured Aquat Resourc, Changsha 410128, Peoples R China
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, Xiudan,Li, Changmei,Lv, Zhao,et al. A calcification-related calmodulin-like protein in the oyster Crassostrea gigas mediates the enhanced calcium deposition induced by CO2 exposure[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,833:11.
APA	Wang, Xiudan,Li, Changmei,Lv, Zhao,Zhang, Zhenqiang,&Qiu, Limei.(2022).A calcification-related calmodulin-like protein in the oyster Crassostrea gigas mediates the enhanced calcium deposition induced by CO2 exposure.SCIENCE OF THE TOTAL ENVIRONMENT,833,11.
MLA	Wang, Xiudan,et al."A calcification-related calmodulin-like protein in the oyster Crassostrea gigas mediates the enhanced calcium deposition induced by CO2 exposure".SCIENCE OF THE TOTAL ENVIRONMENT 833(2022):11.