Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales

doi:10.1016/j.gene.2022.146886

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	Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales
	Yang, Na 1,2,3; Ding, Runting1,2,4 ; Liu, Jianguo 1,2,3
	2022-12-30
发表期刊	GENE
ISSN	0378-1119
卷号	847 页码:9
通讯作者	Liu, Jianguo(jgliu@qdio.ac.cn)
摘要	The accumulation of organic compatible solutes, such as glycine betaine, is one of the osmoprotective strategies used by halophilic archaea to adapt to high salinity. The uptake of glycine betaine from the external environment using various transporters has been widely studied in different halophilic archaea. However, the de novo biosynthesis of glycine betaine and its distribution in halophilic archaea remain unclear. In this study, an extremely halophilic archaea strain, named Halorubrum sp. 2020YC2 and previously isolated from a salt-lake sample, was identified with complete choline oxidation pathway genes. Halorubrum sp. 2020YC2 could syn-thesize and accumulate 1.56-4.25 mu mol per mg of protein of glycine betaine in a defined medium, with its content increasing along with increasing salinity. The intracellular content of glycine betaine remained relatively stable at different salinities when another exogenous solute such as trehalose was provided. The metabolic profile and transcriptional results strongly suggested that the intracellular glycine betaine was derived from serine, which came from the glycolytic intermediate 3-phosphoglycerate when glucose was used as the sole carbon source. Out of 205 available genomes of halophilic archaea, genes encoding the choline oxidation pathway were identified in 30 genomes, and more than half of the strains belonging to order Haloferacales contained the choline oxidation pathway. Phylogenetic analysis further indicated that this pathway evolved from halophilic Proteo-bacteria, and its absence in some genera indicated a possible gene loss event during evolution. The analysis of reported culture data of halophilic archaea strains eventually demonstrated that the presence of the choline oxidation pathway had no significant effects on the adaptation of Haloferacales to high salinity habitats. Therefore, the de novo biosynthesis of glycine betaine via the choline oxidation pathway could be an auxiliary osmoprotective strategy in halophilic archaea.
关键词	Haloferacales Osmoadaptation Glycine betaine Choline oxidation pathway Phylogenetic distribution
DOI	10.1016/j.gene.2022.146886
收录类别	SCI
语种	英语
资助项目	Pilot National Laboratory for Marine Science and Technology (Qingdao), China[YQ2018NO04] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Young Scientists Fund of LMBB
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000867344400001
出版者	ELSEVIER
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180615
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yang, Na,Ding, Runting,Liu, Jianguo. Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales[J]. GENE,2022,847:9.
APA	Yang, Na,Ding, Runting,&Liu, Jianguo.(2022).Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales.GENE,847,9.
MLA	Yang, Na,et al."Synthesizing glycine betaine via choline oxidation pathway as an osmoprotectant strategy in Haloferacales".GENE 847(2022):9.

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