Bis-enzyme cascade CRISPR-Cas12a platform for miRNA detection

doi:10.1016/j.talanta.2022.123837

	Bis-enzyme cascade CRISPR-Cas12a platform for miRNA detection
	Guo, Zixuan 1; Tan, Xiao 2; Yuan, Haoyu 1; Zhang, Ling 1; Wu, Jiajia4 ; Yang, Zhiqing 1; Qu, Ke 3; Wan, Yi 1
	2023-01-15
发表期刊	TALANTA
ISSN	0039-9140
卷号	252 页码:7
通讯作者	Yang, Zhiqing(yangzhiqing@hainanu.edu.cn) ; Qu, Ke(quke18@cdut.edu.cn)
摘要	MicroRNA (miRNA) play a vital role in the pathological development of many diseases. It is considered to be the diagnosis and potential biomarkers of prognosis. Herein, we proposed Bis-enzyme cascade Platform by combining T7 RNA polymerase and CRISPR-Cas12a (BPTC) for a miRNA detection. In the proposed BPTC, the RNA to DNA conversion ability of phi29 amplification and trans -cleavage of CRISPR-Cas12a are combined. The target miRNA can be amplified after binding to the recognizer ssDNA, and then transcribed the CRISPR-derived RNA (crRNA) by T7 RNA polymerase. The produced crRNA can thereby be assembled by CRISPR-Cas12a and recognized with its target dsDNA, thus triggered its trans -cleavage towards surrounding fluorescent reporters, labeled with a fluorophore and a corresponding quenching group. Based on the bis-enzyme cascade system, the biosensor shows highly sensitivity and excellent specificity. Moreover, this study provided a novel all-in-one detect strategy for miRNA and may open a new idea for the design of CRISR-Cas-based miRNA biosensing platforms.
关键词	MicroRNA CRISPR-Cas12a T7 RNA polymerase All -in -one
DOI	10.1016/j.talanta.2022.123837
收录类别	SCI
语种	英语
资助项目	Hainan province key research and development project[ZDYF2021XDNY175] ; Na- tional Natural Science Foundation of China[41866002] ; Na- tional Natural Science Foundation of China[4216060011] ; Hainan Provincial Natural Science Foundation of China[420RC518] ; Open Fund of Shandong Key Laboratory of Corrosion Science[KLCS201910] ; Research Foundation of Hainan University ; KYQD[(ZR) 1711] ; KYQD[(ZR) 1997] ; KYQD[20087] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF04] ; Open fund of Fujian Provincial Key Laboratory of Photonics Tech[JYG 2002] ; Central Government Guides Local Science and Technology Development Projects[ZY2022HN01]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000860344000010
出版者	ELSEVIER
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/180763
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Yang, Zhiqing; Qu, Ke
作者单位	1.Hainan Univ, Marine Coll, State Key Lab Marine Resource Utilizat South China, Haikou 570228, Peoples R China 2.Tsinghua Univ, Dept Chem, Beijing Key Lab Microanalyt Methods & Instrumentat, Beijing 100084, Peoples R China 3.Chengdu Univ Technol, Coll Mat & Chem & Chem Engn, Chengdu 610059, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Guo, Zixuan,Tan, Xiao,Yuan, Haoyu,et al. Bis-enzyme cascade CRISPR-Cas12a platform for miRNA detection[J]. TALANTA,2023,252:7.
APA	Guo, Zixuan.,Tan, Xiao.,Yuan, Haoyu.,Zhang, Ling.,Wu, Jiajia.,...&Wan, Yi.(2023).Bis-enzyme cascade CRISPR-Cas12a platform for miRNA detection.TALANTA,252,7.
MLA	Guo, Zixuan,et al."Bis-enzyme cascade CRISPR-Cas12a platform for miRNA detection".TALANTA 252(2023):7.

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