Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii

doi:10.1093/biolre/ioac203

IOCAS-IR > 实验海洋生物学重点实验室

	Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii
	Wang, Xueying1,2 ; Liu, Qinghua 1,2,5; Li, Jun 1,2,5; Zhou, Li 1,2,3; Wang, Tao 1,2,4; Zhao, Ning 1,2,3
	2022-11-19
发表期刊	BIOLOGY OF REPRODUCTION
ISSN	0006-3363
页码	15
通讯作者	Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
摘要	In S. schlegelii, luteinizing hormone can induce prostanoid production owing to cox upregulation and prostaglandin may be a key pheromone in copulation and sperm maturation. Spermatogenesis is a dynamic cell developmental process that is essential for reproductive success. Vertebrates utilize a variety of reproductive strategies, including sperm diversity, and internal and external fertilization. Research on the cellular and molecular dynamic changes involved in viviparous teleost spermatogenesis, however, is currently lacking. Here, we combined cytohistology, 10 x genomic single-cell RNA-seq, and transcriptome technology to determine the dynamic development characteristics of the spermatogenesis of Sebastes schlegelii. The expressions of lhcgr (Luteinizing hormone/Choriogonadotropin receptor), fshr (follicle-stimulating hormone receptor), ar (androgen receptor), pgr (progesterone receptor), and cox (cyclo-oxygen-ase), as well as the prostaglandin E and F levels peaked during the maturation period, indicating that they were important for sperm maturation and mating. Fifteen clusters were identified based on the 10 x genomic single-cell results. The cell markers of the sub-cluster were identified by their upregulation; piwil, dazl, and dmrt1 were upregulated and identified as spermatogonium markers, and sycp1/3 and spo11 were identified as spermatocyte markers. For S. schlegelii, the sperm head nucleus was elongated (spherical to streamlined in shape), which is a typical characteristic for sperm involved in internal fertilization. We also identified a series of crucial genes associated with spermiogenesis, such as spata6, spag16, kif20a, trip10, and klf10, while kif2c, kifap3, fez2, and spaca6 were found to be involved in nucleus elongation. The results of this study will enrich our cellular and molecular knowledge of spermatogenesis and spermiogenesis in fish that undergo internal fertilization.
关键词	Sebastes schlegelii internal fertilization spermatogenesis spermiogenesis single-cell RNA-seq
DOI	10.1093/biolre/ioac203
收录类别	SCI
语种	英语
WOS研究方向	Reproductive Biology
WOS类目	Reproductive Biology
WOS记录号	WOS:000897040200001
出版者	OXFORD UNIV PRESS INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181017
专题	实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Beijing, Peoples R China 4.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Xueying,Liu, Qinghua,Li, Jun,et al. Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii[J]. BIOLOGY OF REPRODUCTION,2022:15.
APA	Wang, Xueying,Liu, Qinghua,Li, Jun,Zhou, Li,Wang, Tao,&Zhao, Ning.(2022).Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii.BIOLOGY OF REPRODUCTION,15.
MLA	Wang, Xueying,et al."Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii".BIOLOGY OF REPRODUCTION (2022):15.

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