The preliminary DNA barcoding dataset of fishes from Chinese coastal waters

doi:10.1016/j.rsma.2022.102699

IOCAS-IR > 实验海洋生物学重点实验室

	The preliminary DNA barcoding dataset of fishes from Chinese coastal waters
	Wang, Lijuan1,2 ; Wu, Zhihao 1,2; Sun, Guiqing 3; Chen, Hui 4; Liu, Hongjun 5; You, Feng 1,2
	2022-11-01
发表期刊	REGIONAL STUDIES IN MARINE SCIENCE
ISSN	2352-4855
卷号	56 页码:9
通讯作者	You, Feng(youfeng@qdio.ac.cn)
摘要	DNA barcoding is a molecular method for species identification based on a short standardized DNA sequence derived from the 5' region of the cytochrome c oxidase subunit I (COI) gene, which is widely applied in marine fish identification. In this study, DNA barcodes were generated for 1,671 specimens representing 309 morphological marine fish species belonging to 223 genera and covering 101 families from Chinese coastal waters. The mean Kimura 2-parameter (K2P) genetic distances within species, genera, families, and orders were 0.20%, 20.94%, 24.45%, and 25.34%, respectively. As expected, the mean interspecific distance was higher than the mean intraspecific distance. Phylogenetic trees based on Bayesian inference (BI) and neighbor-joining (NJ) methods agreed with the taxonomic classifications. A total of 306 operational taxonomic units (OTUs) were reidentified via automatic barcode gap discovery (ABGD) analysis. An estimated nucleotide substitution matrices showed a transversion bias compared to transitional mutation. The amino acid variations of the barcoding region were also examined, and 13 amino acid sites exhibited high variability. Overall, our findings confirmed that DNA barcoding is a highly reliable mean of identifying fish species in Chinese coastal waters, and that the barcode library established here would facilitate the monitoring and management of the marine fishery resources in China. (C) 2022 Elsevier B.V. All rights reserved.
关键词	Cytochrome c oxidase subunit I (COI) DNA barcoding Fish identification Biodiversity Amino acid variation China
DOI	10.1016/j.rsma.2022.102699
收录类别	SCI
语种	英语
资助项目	Special Program for Basic Research of the Ministry of Science and Technology of China[2014FY110500] ; Key Research and Development Program of Shandong Province, China[2021LZGC029] ; Major Agricultural Application Technology and Innovation Projects of Shandong Province, China (2017-2020) ; National Marine Genetic Resource Center of China
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Ecology ; Marine & Freshwater Biology
WOS记录号	WOS:000901657300005
出版者	ELSEVIER
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/181074
专题	实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Hebei Ocean & Fisheries Sci Res Inst, Qinhuangdao 066000, Hebei, Peoples R China 4.State Ocean Adm, Isl Res Ctr, Pingtan 350400, Peoples R China 5.Marine Sci Res Inst Shandong Prov, Qingdao 266002, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Lijuan,Wu, Zhihao,Sun, Guiqing,et al. The preliminary DNA barcoding dataset of fishes from Chinese coastal waters[J]. REGIONAL STUDIES IN MARINE SCIENCE,2022,56:9.
APA	Wang, Lijuan,Wu, Zhihao,Sun, Guiqing,Chen, Hui,Liu, Hongjun,&You, Feng.(2022).The preliminary DNA barcoding dataset of fishes from Chinese coastal waters.REGIONAL STUDIES IN MARINE SCIENCE,56,9.
MLA	Wang, Lijuan,et al."The preliminary DNA barcoding dataset of fishes from Chinese coastal waters".REGIONAL STUDIES IN MARINE SCIENCE 56(2022):9.

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