Optimizing fucoxanthin production from Phaeodactylum tricornutum: Impact of harvesting methods on culture medium reusability

doi:10.1016/j.algal.2023.103150

IOCAS-IR > 实验海洋生物学重点实验室

	Optimizing fucoxanthin production from Phaeodactylum tricornutum: Impact of harvesting methods on culture medium reusability
	Wu, Songcui1,2 ; Gu, Wenhui 1,2; Jia, Shuao 1,2,3; Xie, Xiujun 1,2; Wang, Lijun 1,2; Wang, Guangce 1,2
	2023-07-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	74 页码:12
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
摘要	The growing demand for naturally-synthesized fucoxanthin has driven increased focus on microalgae-based production methods. However, the high costs associated with microalgae harvesting and processing remain a significant challenge. To overcome this obstacle and promote the development of the microalgae-based fuco-xanthin industry, this study aimed to develop an efficient and cost-effective methods for harvesting Phaeo-dactylum tricornutum, a promising microalgal strain for fucoxanthin production, while maximizing the reuse of the culture medium. This study investigated the feasibility of reusing the FeCl3-based flocculation medium for P. tricornutum cultivation, and explored the optimal proportion of recycled supernatant and fresh medium for maximizing biomass and fucoxanthin yield of P. tricornutum. It also examined the effect of residual iron on harvested biomass and pigment and proposed a simple washing method for its removal. The results showed that FeCl3-based flocculation was highly effective (>93 %) in harvesting P. tricornutum, and a 50/50 proportion of recycled medium and fresh medium can be used without significant negative effects on biomass and fucoxanthin productivity, allowing a reduction of approximately 43 % water consumption. Although a negative effect on algal fucoxanthin content was observed, the productivity of fucoxanthin per unit volume (mg/L) did not decrease compared to centrifugation-based algae cells. Furthermore, simple washing with water can effectively remove the residual iron from the harvested biomass or pigment, resulting in a reduction of approximately 42 % and 64 %, respectively. These findings suggest that the reuse of FeCl3-based flocculation medium for P. tricornutum cultivation is feasible and holds promise for cost-effective fucoxanthin production. This study contributes to develop a sustainable approach for microalgae-based fucoxanthin production at a lower cost and higher pro-duction capacity, benefiting both society and industry.
关键词	Cost-effective production Culture medium recycling Fucoxanthin Harvesting methods Microalgae-based production Phaeodactylum tricornutum
DOI	10.1016/j.algal.2023.103150
收录类别	SCI
语种	英语
资助项目	Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; National Natural Science Foundation of China[42276146] ; Key Deployment Project of the Centre for Ocean Mega -Research of Science of Chinese Academy of Sciences[COMS2019Q02]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:001022965600001
出版者	ELSEVIER
WOS关键词	CHLORELLA-VULGARIS ; ALKALINE-PHOSPHATASE ; LIPID-ACCUMULATION ; MICROALGAE ; BIOMASS ; FLOCCULATION ; CULTIVATION ; REMOVAL ; GROWTH ; IRON
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182458
专题	实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wu, Songcui,Gu, Wenhui,Jia, Shuao,et al. Optimizing fucoxanthin production from Phaeodactylum tricornutum: Impact of harvesting methods on culture medium reusability[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2023,74:12.
APA	Wu, Songcui,Gu, Wenhui,Jia, Shuao,Xie, Xiujun,Wang, Lijun,&Wang, Guangce.(2023).Optimizing fucoxanthin production from Phaeodactylum tricornutum: Impact of harvesting methods on culture medium reusability.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,74,12.
MLA	Wu, Songcui,et al."Optimizing fucoxanthin production from Phaeodactylum tricornutum: Impact of harvesting methods on culture medium reusability".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 74(2023):12.