LvCD14L Acts as a Novel Pattern Recognition Receptor and a Regulator of the Toll Signaling Pathway in Shrimp

doi:10.3390/ijms24097770

IOCAS-IR > 实验海洋生物学重点实验室

	LvCD14L Acts as a Novel Pattern Recognition Receptor and a Regulator of the Toll Signaling Pathway in Shrimp
	Lv, Xinjia1 ; Li, Shihao 1,2,3; Yu, Yang1,2,3 ; Jin, Songjun 1,2,3; Zhang, Xiaojun 1,2,3; Li, Fuhua 1,2,3,4
	2023-04-24
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	24 期号:9 页码:16
通讯作者	Li, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
摘要	Leucine-rich repeat (LRR) is a structural motif has important recognition function in immune receptors, such as Tolls and NOD-like receptors (NLRs). The immune-related LRR proteins can be divided into two categories, LRR-containing proteins and LRR-only proteins. The latter contain LRR motifs while they are without other functional domains. However, the functional mechanisms of the LRR-only proteins were still unclear in invertebrates. Here, we identified a gene encoding a secretory LRR-only protein, which possessed similarity with vertebrate CD14 and was designated as LvCD14L, from the Pacific whiteleg shrimp Litopenaeus vannamei. Its transcripts in shrimp hemocytes were apparently responsive to the infection of Vibrio parahaemolyticus. Knockdown of LvCD14L with dsRNA resulted in significant increase of the viable bacteria in the hepatopancreas of shrimp upon V. parahaemolyticus infection. Further functional studies revealed that LvCD14L could bind to microorganisms' PAMPs, showed interaction with LvToll1 and LvToll2, and regulated the expression of LvDorsal and LvALF2 in hemocytes. These results suggest that LvCD14L functions as a pattern recognition receptor and activates the NF-?B pathway through interaction with LvTolls. The present study reveals a shrimp LvCD14L-Tolls-NF-?B signaling pathway like the CD14/TLR4/NF-?B signaling pathway in mammalians, which enriches the functional mechanism of secretory LRR-only immune receptors during pathogens infection in invertebrates.
关键词	pattern recognition receptor CD14 toll NF-kappa B pathway Vibrio parahaemolyticus Litopenaeus vannamei
DOI	10.3390/ijms24097770
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[31972829] ; Taishan Scholars Program ; earmarked fund for CARS-48
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000987515300001
出版者	MDPI
WOS关键词	LEUCINE-RICH REPEAT ; RNAI KNOCK-DOWN ; LITOPENAEUS-VANNAMEI ; INNATE IMMUNITY ; PROTEIN ; CD14 ; PENTRAXINS ; BINDING ; GENE ; INFLAMMATION
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183117
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Lv, Xinjia,Li, Shihao,Yu, Yang,et al. LvCD14L Acts as a Novel Pattern Recognition Receptor and a Regulator of the Toll Signaling Pathway in Shrimp[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(9):16.
APA	Lv, Xinjia,Li, Shihao,Yu, Yang,Jin, Songjun,Zhang, Xiaojun,&Li, Fuhua.(2023).LvCD14L Acts as a Novel Pattern Recognition Receptor and a Regulator of the Toll Signaling Pathway in Shrimp.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(9),16.
MLA	Lv, Xinjia,et al."LvCD14L Acts as a Novel Pattern Recognition Receptor and a Regulator of the Toll Signaling Pathway in Shrimp".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.9(2023):16.