Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus)

doi:10.1016/j.scitotenv.2022.160430

IOCAS-IR > 实验海洋生物学重点实验室

	Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus)
	Qi, Ting 1; Wu, Lele1 ; Yu, Jiachen 3; Song, Zongcheng 4; Liu, Feng 2; Li, Jun 2; Song, Xiefa 1; Li, Xian 1
	2023-02-25
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
卷号	861 页码:12
通讯作者	Li, Xian(lixian@ouc.edu.cn)
摘要	Phosphate, as the main nutrient factor of lake eutrophication brought by pollutants discharged from agriculture and industry, is always considered to be a low-toxicity substance to aquatic animals. But the toxicity mechanism is unclear, and published information is limited. In this study, a 96 h acute stress experiment was conducted on juvenile turbot (Scophthalmus maximus) with 0, 10, and 60 mg/L phosphate solutions. Metabonomic analysis revealed that low -dose phosphate (10 mg/L) disrupted glycerophospholipid, purine, and glycolipid metabolism, as well as the tricarbox-ylic acid (TCA) cycle in juveniles, even at 96 h of stress, which may lead to cell structure damage and signal recognition disorder between cells. Upregulated key genes in the main glycerophospholipid metabolic pathways, which matched the results of the metabolomic study, were detected. Furthermore, low-dose phosphate (10 mg/L) induced oxidative stress and immunotoxicity in fish, resulting in the raising of relevant genes expression such as cat and sod in liver and kidney. In addition, all phosphate-treated groups had induced lesions on gill tissue, as evidenced by pathological observations. In this study on toxic effects on and mechanism of phosphate in aquatic animals using metabolomics, gene expression, and histopathology, we confirm that acute low-dose phosphate could disrupt glycerophospholipid metabolism and induce stress in juvenile turbot. This can provide advice on the amount of phosphate accumulation for marine fish farming and on protecting species diversity and marine ecosystem from the point of view of phosphate toxicity to marine animals.
关键词	Phosphate Toxicity Metabolomics Histopathological damage Gene expression
DOI	10.1016/j.scitotenv.2022.160430
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42061160365] ; National Natural Science Foundation of China[41976122] ; Young Talent Program of Ocean University of China[862201013156] ; Science and Technology Development Planning Projects of Huancui ; China Agriculture Research System[CARS -47]
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000901627600013
出版者	ELSEVIER
WOS关键词	ACTIVATED PROTEIN-KINASE ; IMMUNE-RESPONSES ; STOCKING DENSITY ; CELLULAR-ENERGY ; MYOSIN-II ; WATER ; HEALTH ; EUTROPHICATION ; PERFORMANCE ; EXPRESSION
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183282
专题	实验海洋生物学重点实验室
通讯作者	Li, Xian
作者单位	1.Ocean Univ China, Fisheries Coll, Key Lab Mariculture, Minist Educ, Qingdao 266001, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Jiangsu Ocean Univ, Sch Marine Sci & Fisheries, Lianyungang 222005, Jiangsu, Peoples R China 4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
推荐引用方式 GB/T 7714	Qi, Ting,Wu, Lele,Yu, Jiachen,et al. Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus)[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,861:12.
APA	Qi, Ting.,Wu, Lele.,Yu, Jiachen.,Song, Zongcheng.,Liu, Feng.,...&Li, Xian.(2023).Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus).SCIENCE OF THE TOTAL ENVIRONMENT,861,12.
MLA	Qi, Ting,et al."Acute low-dose phosphate disrupts glycerophospholipid metabolism and induces stress in juvenile turbot (Scophthalmus maximus)".SCIENCE OF THE TOTAL ENVIRONMENT 861(2023):12.