Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction

doi:10.1021/acs.analchem.2c04484

	Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction
	Zhou, Yanmei 1,2,3; Xie, Shupu 1; Liu, Bo 1; Wang, Cong 1; Huang, Yibo 1; Zhang, Xiaoru 1,4; Zhang, Shusheng 5,6
	2023-01-30
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
页码	8
通讯作者	Zhang, Xiaoru(zhangxr7407@126.com) ; Zhang, Shusheng(shushzhang@126.com)
摘要	Herein, a chemiluminescence (CL) biosensor based on CRISPR-Cas12a and cation exchange reaction was constructed to detect the biomarker microRNA-21 (miRNA-21). The rolling circle amplification (RCA) reaction was introduced to convert each target RNA strand into a long single-stranded DNA with repeated sequences, which acted as triggers to initiate the transcleavage activity of CRISPR-Cas12a. The activated Cas12a could cleave the biotinylated linker DNA of CuS nanoparticles (NPs) to inhibit the binding of CuS NPs to streptavidin immobilized on the surface of the microplate, which strongly reduced the generation of Cu2+ from a cation exchange between CuS NPs and AgNO3, and thus efficiently suppressed the CL of Cu2+-luminol-H2O2 system, giving a "signal off" biosensor. With the multiple amplification, the detection limit of the developed sensor for miRNA-21 reached 16 aM. In addition, this biosensor is not only suitable for a professional chemiluminescence instrument but also for a smartphone used as a detection tool for the purpose of portable and low-cost assay. This method could be used to specifically detect quite a low level of miRNA-21 in human serum samples and various cancer cells, indicating its potential in ultrasensitive molecular diagnostics.
DOI	10.1021/acs.analchem.2c04484
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[22276103] ; National Natural Science Foundation of China[22274068] ; National Natural Science Foundation of China[22076073]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000925401700001
出版者	AMER CHEMICAL SOC
WOS关键词	MICRORNA EXPRESSION ; BIOLOGICAL CONSTITUENT ; AMPLIFICATION
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/183450
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Xiaoru; Zhang, Shusheng
作者单位	1.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Qingdao Univ Sci & Technol, Key Lab Opt Elect Sensing & Analyt Chem Life Sci, Shandong Key Lab Biochem Anal, MOE, Qingdao 266042, Peoples R China 5.Equipment & Diag Therapy Integrat Univ Shandong, Linyi Univ, Coll Chem & Chem Engn, Collaborat Innovat Ctr Tumor Marker Detect Technol, Linyi 276000, Peoples R China 6.Linyi Univ, Coll Chem & Chem Engn, Linyi 276000, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Zhou, Yanmei,Xie, Shupu,Liu, Bo,et al. Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction[J]. ANALYTICAL CHEMISTRY,2023:8.
APA	Zhou, Yanmei.,Xie, Shupu.,Liu, Bo.,Wang, Cong.,Huang, Yibo.,...&Zhang, Shusheng.(2023).Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction.ANALYTICAL CHEMISTRY,8.
MLA	Zhou, Yanmei,et al."Chemiluminescence Sensor for miRNA-21 Detection Based on CRISPR-Cas12a and Cation Exchange Reaction".ANALYTICAL CHEMISTRY (2023):8.