Characteristics of <i>Shisa</i> Family Genes in Zebrafish

doi:10.3390/ijms241814062

IOCAS-IR > 实验海洋生物学重点实验室

	*Characteristics of Shisa* Family Genes in Zebrafish**
	Liu, Yansong 1,2,3; Du, Na2,3,4 ; Qian, Beibei 2,3; Zou, Congcong2,3,4 ; Yu, Zhouxin 1,2,3; Xu, Fei 2,3; Wang, Lijuan2,3 ; Qin, Sishi 2,3,4; You, Feng 2,3; Tan, Xungang2,3
	2023-09-01
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
卷号	24 期号:18 页码:17
通讯作者	Tan, Xungang(tanx@qdio.ac.cn)
摘要	Shisa represents a type of single-transmembrane adaptor protein containing an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Nine shisa subfamily genes have been proposed in most vertebrates; however, some might be species-specific. The number of shisa genes present in zebrafish remains unclear. This study aimed to investigate the evolutionary relationships among shisa family genes in zebrafish (TU strain) using phylogenetic and syntenic analyses. The function of shisa-2 was preliminarily examined via CRISPR/Cas13d-mediated knockdown. Following identification in zebrafish, 10 shisa family genes, namely shisa-1, 2, 3, 4, 5, 6, 7, 8, 9a, and 9b, were classified into three main clades and six subclades. Their encoding proteins contained a cysteine-rich N-terminal domain and a proline-rich C-terminal region containing different motifs. A specific syntenic block containing atp8a2 and shisa-2 was observed to be conserved across all species. Furthermore, all these genes were expressed during embryogenesis. Shisa-2 was expressed in the presomitic mesoderm, somites, and so on. Shisa-2 was identified as a regulator of the expression of the somite formation marker mesp-ab. Overall, our study provides new insights into the evolution of shisa family genes and the control of shisa-2 over the convergent extension cells of somitic precursors in zebrafish.
关键词	gene expression knockdown CRISPR/Cas13d shisa-2 mesp-ab somite formation
DOI	10.3390/ijms241814062
收录类别	SCI
语种	英语
资助项目	Shandong Provincial Natural Science Foundation
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:001122207400001
出版者	MDPI
WOS关键词	EXPRESSION ; PROTEINS ; RECEPTOR ; WNT ; MATURATION ; PLASTICITY ; DIVERSITY ; HOMOLOGS ; PROMOTES ; PAX3
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184114
专题	实验海洋生物学重点实验室
通讯作者	Tan, Xungang
作者单位	1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 2.Inst Oceanol, Chinese Acad Sci, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Lab Marine Biol & Biotechnol, Pilot Natl Lab Marine Sci & Technol Qingdao, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Yansong,Du, Na,Qian, Beibei,et al. Characteristics of Shisa Family Genes in Zebrafish[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(18):17.
APA	Liu, Yansong.,Du, Na.,Qian, Beibei.,Zou, Congcong.,Yu, Zhouxin.,...&Tan, Xungang.(2023).Characteristics of Shisa Family Genes in Zebrafish.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(18),17.
MLA	Liu, Yansong,et al."Characteristics of Shisa Family Genes in Zebrafish".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.18(2023):17.