Metabolic proteins with crucial roles in <i>Edwardsiella tarda</i> antioxidative adaptation and intracellular proliferation

doi:10.1128/msystems.00391-23

IOCAS-IR > 实验海洋生物学重点实验室

	*Metabolic proteins with crucial roles in Edwardsiella tarda* antioxidative adaptation and intracellular proliferation**
	Wang, Xinhui 1,2; Sun, Boguang1,2,3
	2023-09-20
发表期刊	MSYSTEMS
ISSN	2379-5077
页码	15
通讯作者	Sun, Boguang(sunboguang@qdio.ac.cn)
摘要	Edwardsiella tarda is a severe fish pathogen, featured by its capacity to live inside host phagocytes. For intracellular survival, it is crucial for E. tarda to neutralize the deleterious effect of host reactive oxygen species (ROS). Accumulating evidence suggests that bacterial metabolism is closely connected to oxidative resistance. However, the roles of E. tarda metabolic proteins in antioxidative adaptation and intracellular proliferation remain elusive. In this study, we performed a proteomic analysis on E. tarda and identified 111 proteins responsive to H2O2-mediated oxidative stress. Based on this data, we further obtained eight crucial proteins, including seven metabolic proteins, for E. tarda antioxidation and intracellular infection. Among them, two C4-dicarboxylate transporters were found necessary for E. tarda to disseminate in fish tissues. Furthermore, the substrate of the two transporters was identified as L-aspartate, which was proven to be essential for the full antioxidative capacity of E. tarda. Our results indicate that reprogramming the metabolic flux to the production of pyruvate, a ketoacid capable of neutralizing ROS, was likely a pivotal strategy of E. tarda to survive the oxidative environments inside host cells. Together, the findings of this study highlight the significance of metabolic reprogramming for bacterial redox homeostasis and intracellular infection.
关键词	Edwardsiella tarda oxidative stress virulence metabolic reprogramming proteomics
DOI	10.1128/msystems.00391-23
收录类别	SCI
语种	英语
资助项目	The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001145055800001
出版者	AMER SOC MICROBIOLOGY
WOS关键词	OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; PHAGOCYTES ; RESISTANCE ; PATHOGENESIS ; MECHANISMS ; KATB
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184366
专题	实验海洋生物学重点实验室
通讯作者	Sun, Boguang
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao, Peoples R China 3.Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, Xinhui,Sun, Boguang. Metabolic proteins with crucial roles in Edwardsiella tarda antioxidative adaptation and intracellular proliferation[J]. MSYSTEMS,2023:15.
APA	Wang, Xinhui,&Sun, Boguang.(2023).Metabolic proteins with crucial roles in Edwardsiella tarda antioxidative adaptation and intracellular proliferation.MSYSTEMS,15.
MLA	Wang, Xinhui,et al."Metabolic proteins with crucial roles in Edwardsiella tarda antioxidative adaptation and intracellular proliferation".MSYSTEMS (2023):15.