Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90

doi:10.1016/j.cej.2024.148590

	Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90
	Wang, Yingwen 1,2; Qi, Peng1,3 ; Zhang, Dun 1,3; Zeng, Yan1,3 ; Sun, Yan 1,3; Wang, Peng1,3
	2024-02-01
发表期刊	CHEMICAL ENGINEERING JOURNAL
ISSN	1385-8947
卷号	481 页码:9
通讯作者	Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) ; Wang, Peng(wangpeng@qdio.ac.cn)
摘要	Adenosine triphosphate (ATP) is an important biological biomarker, but its rapid extracellular decomposition poses challenges for on-site detection. We report a paper-based dual-readout viscosity flow sensor enabling rapid, equipment-free quantification of ATP. The assay integrates molybdenum disulfide nanozymes for signal amplification with zeolitic imidazolate framework-90 (ZIF-90) for selective ATP recognition and nanozyme regulation. In the presence of ATP, the intact chitosanase-encapsulated ZIF-90 structure was disintegrated to release chitosanase and thus hydrolyzed chitosan hydrogel, decreasing the viscosity of chitosan solution. Moreover, elimination of ZIF-90 inhibitory effect reactivated the catalytic activity of MoS2 nanozyme, resulting in the oxidation of 3,3 ',5,5 '-tetramethylbenzidine (TMB) to produce obvious color change. The analytical signals, combining the distance- and colorimetric- readout, were directly displayed on filter paper without further processed. The proposed dual-readout viscosity flow sensor exhibited remarkable sensitivity for ATP detection with detection limits down to 0.038 nM with the distance-readout and 0.057 nM for the colorimetric-readout. Furthermore, we developed an integrated paper kit with a printed scale and color card for standardized on-site analysis without instrumentation. Successful application for ATP quantification in bacterial biofilms and blood samples demonstrates the sensor's strong performance and potential for decentralized testing.
关键词	Paper-based ATP assay Degradation of high viscosity hydrogel Nanozyme reactivation Onsite detection ZIF-90
DOI	10.1016/j.cej.2024.148590
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42376208]
WOS研究方向	Engineering
WOS类目	Engineering, Environmental ; Engineering, Chemical
WOS记录号	WOS:001161457900001
出版者	ELSEVIER SCIENCE SA
WOS关键词	PATHOGENS ; ANTIBODY ; ACID
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184442
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Qi, Peng; Zhang, Dun; Wang, Peng
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Chinese Acad Agr Sci, Key Lab Tobacco Pest Monitoring & Integrated Manag, Tobacco Res Inst, Qingdao 266101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Yingwen,Qi, Peng,Zhang, Dun,et al. Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90[J]. CHEMICAL ENGINEERING JOURNAL,2024,481:9.
APA	Wang, Yingwen,Qi, Peng,Zhang, Dun,Zeng, Yan,Sun, Yan,&Wang, Peng.(2024).Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90.CHEMICAL ENGINEERING JOURNAL,481,9.
MLA	Wang, Yingwen,et al."Dual-signal viscosity flow paper sensor for ATP detection based on bio-recognition and nanozyme activity regulation of ZIF-90".CHEMICAL ENGINEERING JOURNAL 481(2024):9.