DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process

doi:10.1016/j.aquaculture.2024.740595

IOCAS-IR > 实验海洋生物学重点实验室

	DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process
	Wang, Xinxing1,2,3,5 ; Wei, Yuheng 4; Cong, Rihao 1,2,5,7,8,10; Wang, Wei 1,2,6,8; Qi, Haigang 1,2,5,7,8; Zhang, Guofan 1,2,5,7,9; Pan, Ying 4,12; Li, Li 1,2,6,7,8,9,11
	2024-03-30
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	583 页码:9
通讯作者	Pan, Ying(yingpan@gxu.edu.cn) ; Li, Li(lili@qdio.ac.cn)
摘要	Excessive energy consumption during reproductive process seriously reduces the nutritive value of oysters. Significant reduction of meat quality was detected in condition index and glycogen content in postspawning oysters. In order to investigate the molecular mechanism underlying this variation in quality, a conjoint analysis of DNA methylation and RNA sequencing was conducted in this study. Oysters exhibited divergent gene expression profiles during reproduction, with a particular acceleration observed in glycogen and fatty acid metabolism related pathways in postspawning oysters, including glycolysis/gluconeogenesis, TCA cycle and fatty acid degradation. Changes in genomic DNA methylation between prespawning and postspawning oysters were also detected, with oysters after spawning exhibited increased methylation trend in promoter, genebody and downstream regions. Significantly, differently methylated genes were enriched in AMPK signal pathway, and differently methylated sites of AMPK alpha were identified. Combined with the gene expression variation in energy metabolism network dominated by AMPK, it can be inferred that DNA methylation could regulate energy metabolism homeostasis through influencing the gene expression of AMPK signal pathway. These findings suggested that DNA methylation-mediated energy metabolism plays a crucial role in regulating oyster glycogen content and meat quality during reproduction. This study revealed the epigenetic mechanism of quality variation during oyster reproduction for the first time.
关键词	Oysters Quality losses Energy metabolism DNA methylation Reproductive process AMPK signal pathway
DOI	10.1016/j.aquaculture.2024.740595
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42306108] ; National Key Research and Development Program of China[2022YFD2400301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050402] ; Key Research and Development Program of Shandong[2022LZGC015] ; China Agriculture Research System of MOF and MARA[CARS -49] ; Key Technology Research and Industrialization Demonstration Projects of Qingdao[22-3-3-hygg-2-hy]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001172925300001
出版者	ELSEVIER
WOS关键词	CRASSOSTREA-GIGAS ; CONDITION INDEX ; IDENTIFICATION ; EPIGENETICS ; PATHWAY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184787
专题	实验海洋生物学重点实验室
通讯作者	Pan, Ying; Li, Li
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Shandong Univ, Sch Life Sci, Qingdao 266237, Peoples R China 4.Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Peoples R China 5.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 8.Shandong Technol Innovat Ctr Oyster Seed Ind, Qingdao 266000, Peoples R China 9.Chinese Acad Sci, Inst Hydrobiol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 10.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China 11.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 12.Guangxi Univ, Coll Anim Sci & Technol, Nanning, Guangxi, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Xinxing,Wei, Yuheng,Cong, Rihao,et al. DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process[J]. AQUACULTURE,2024,583:9.
APA	Wang, Xinxing.,Wei, Yuheng.,Cong, Rihao.,Wang, Wei.,Qi, Haigang.,...&Li, Li.(2024).DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process.AQUACULTURE,583,9.
MLA	Wang, Xinxing,et al."DNA methylation-mediated energy metabolism provides new insight into the quality losses during oyster reproductive process".AQUACULTURE 583(2024):9.