Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from<i> Litopenaeus</i><i> vannamei</i>

doi:10.1016/j.dci.2024.105146

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from Litopenaeus vannamei
	Hu, Jie 1,2,3; Li, Shihao 1,2,4,5; Miao, Miao 1,2,6; Li, Fuhua 1,2,4,5,7
	2024-05-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	154 页码:9
通讯作者	Li, Shihao(lishihao@qdio.ac.cn)
摘要	Microbial drug resistance is becoming increasingly severe due to antibiotic abuse. The development and utilization of antimicrobial peptides is one of the important ways to solve this difficult problem. Crustins are a family of antimicrobial peptides that play important roles in the innate immune system of crustaceans. Several types of crustins exist in shrimp and their activities vary greatly. In the present study, we studied the immune function of one newly identified crustin and found that the type VI crustin encoding gene in Litopenaeus vannamei (LvCrustinVI) was mainly expressed in gills. Its expression was significantly up-regulated after Vibrio parahaemolyticus infection and knockdown of the gene promoted Vibrio proliferation in the hepatopancreas of shrimp, indicating that LvCrustinVI was involved in pathogens infection. The recombinant LvCrustinVI (rLvCrustinVI) showed strong inhibitory activities against both Gram-negative and Gram-positive bacteria, and exhibited binding activities with the bacteria and bacterial polysaccharides including Glu, LPS and PGN. In the presence of Ca2+, rLvCrustinVI showed a strong agglutination effect on V. parahaemolyticus and could significantly enhance the phagocytic ability of shrimp hemocytes against V. parahaemolyticus. In conclusion, LvCrustinVI played important roles as antimicrobial peptide and opsonin in the innate immune defense of L. vannamei. The study enriched our understanding of the functional activity of Crustin and provides an important basis for the development and utilization of antimicrobial peptides.
关键词	Crustin Antibacterial activity Agglutination Phagocytic ability Opsonin
DOI	10.1016/j.dci.2024.105146
收录类别	SCI
语种	英语
资助项目	Key Program of National Natural Science Foundation of China[31830100] ; General Program of National Natural Science Foundation of China[31972829] ; Earmarked fund for CARS-48 ; Taishan Scholars Program
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS类目	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS记录号	WOS:001186776400001
出版者	ELSEVIER SCI LTD
WOS关键词	DOMAIN-CONTAINING PROTEIN ; MUD CRAB ; CRUSTIN ; IMMUNITY ; BINDING
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184796
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Ocean Univ China, Key Lab Marine Environm & Ecol, Minist Educ, Qingdao 266100, Peoples R China 4.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Jie,Li, Shihao,Miao, Miao,et al. Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2024,154:9.
APA	Hu, Jie,Li, Shihao,Miao, Miao,&Li, Fuhua.(2024).Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,154,9.
MLA	Hu, Jie,et al."Characterization of the antibacterial and opsonic functions of the antimicrobial peptide LvCrustinVI from Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 154(2024):9.