Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco

doi:10.3389/fmicb.2024.1361883

	Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco
	Yang, Luhua 1,2; Guo, Yuan 3; Yang, Hui3,4,5 ; Li, Shun 2,6; Zhang, Yunzeng 7; Gao, Cheng 8; Wei, Tian 9; Hao, Likai 3,5,10
	2024-03-01
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	15 页码:13
通讯作者	Hao, Likai(haolikai@mail.gyig.ac.cn)
摘要	The plant microbiota is believed to be an accessory genome that extends plant functions, forming holobionts together with the host plant. Plant disease resistance, therefore, is inextricably linked with plant microbiota, which play important roles in plant growth and health. To explore the relationship between plant microbiota and disease resistance, we investigated the tobacco microbiome of two varieties with contrasting disease-resistance levels to bacterial wilt and black shank diseases. Comparative microbiome analysis indicated that the resistant variety assembled a distinct microbiota with higher network complexity and diversity. While Pseudomonas and Ensifer, which contain biocontrol and beneficial members, were enriched in the rhizosphere of the resistant variety, Ralstonia, a genus including the known causative pathogen, was enriched in the susceptible variety. Metagenome sequencing revealed that biocontrol functions, such as hydrogen cyanide synthase, pyochelin biosynthesis, and arthrofactin-type cyclic lipopeptide synthetase, were more abundant in the resistant variety. Further analysis indicated that contigs encoding the corresponding genes were mostly assigned to Pseudomonas. Among all the metagenome-assembled genomes, positive selection was suggested in the genome assigned to Pseudomonas only in the rhizosphere of the resistant variety. The search of biosynthetic gene clusters in the Pseudomonas genome revealed a non-ribosomal peptide synthetase, the compound of which was brabantamide A, with known antimicrobial activity. Collectively, our study suggests that the plant microbiota might be involved in microbe-mediated disease resistance. Particularly, our results highlight Pseudomonas in the rhizosphere of the disease-resistant variety as a promising biocontrol candidate. Our study may facilitate further screening of bacterial isolates and the targeted design of microbial communities.
关键词	plant microbiota tobacco microbiome disease resistance positive selection biocontrol
DOI	10.3389/fmicb.2024.1361883
收录类别	SCI
语种	英语
资助项目	Guizhou Provincial Department of Science and Technology
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001185098200001
出版者	FRONTIERS MEDIA SA
WOS关键词	PATHOGEN ; PSEUDOMONAS ; DIVERSITY ; BACTERIAL ; INHIBITORS ; SB-253514 ; STRAIN ; GROWTH ; SOIL
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184817
专题	海洋环境腐蚀与生物污损重点实验室
通讯作者	Hao, Likai
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Ningbo Urban Environm Observat & Res Stn, Xiamen, Peoples R China 3.Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang, Peoples R China 4.Guizhou Acad Tobacco Sci, Guiyang, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China 6.CAS Haixi Ind Technol Innovat Ctr Beilun, Zhejiang Key Lab Urban Environm Proc & Pollut Cont, Ningbo 315800, Peoples R China 7.Yangzhou Univ, Coll Biosci & Biotechnol, Yangzhou, Peoples R China 8.Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing, Peoples R China 9.Bei Bu Zhan Qu CDC, Shenyang, Peoples R China 10.CAS Ctr Excellence Quaternary Sci & Global Change, Xian, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yang, Luhua,Guo, Yuan,Yang, Hui,et al. Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco[J]. FRONTIERS IN MICROBIOLOGY,2024,15:13.
APA	Yang, Luhua.,Guo, Yuan.,Yang, Hui.,Li, Shun.,Zhang, Yunzeng.,...&Hao, Likai.(2024).Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco.FRONTIERS IN MICROBIOLOGY,15,13.
MLA	Yang, Luhua,et al."Distinct microbiota assembly and functional patterns in disease-resistant and susceptible varieties of tobacco".FRONTIERS IN MICROBIOLOGY 15(2024):13.