<i>Bacillus cereus</i> cereolysin O induces pyroptosis in an undecapeptide-dependent manner

doi:10.1038/s41420-024-01887-7

IOCAS-IR > 实验海洋生物学重点实验室

	Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner
	Wang, Yujian1,2,3 ; Luo, Jingchang1,2,3,4 ; Guan, Xiaolu 1,2,3,4; Zhao, Yan5 ; Sun, Li 1,2,3,4
	2024-03-08
发表期刊	CELL DEATH DISCOVERY
卷号	10 期号:1 页码:9
通讯作者	Zhao, Yan(zhaoyan1316@163.com) ; Sun, Li(lsun@qdio.ac.cn)
摘要	Bacillus cereus is a clinically significant foodborne pathogen that causes severe gastrointestinal and non-gastrointestinal disease. Cereolysin O (CLO) is a putative virulence factor of B. cereus, and its function remains to be investigated. In this study, we examined the biological activity of CLO from a deep sea B. cereus isolate. CLO was highly toxic to mammalian cells and triggered pyroptosis through NLRP3 inflammasome-mediated caspase 1 and gasdermin D activation. CLO-induced cell death involved ROS accumulation and K+ efflux, and was blocked by serum lipids. CLO bound specifically to cholesterol, and this binding was essential to CLO cytotoxicity. The structural integrity of the three tryptophan residues in the C-terminal undecapeptide was vital for CLO to interact with membrane lipids and cause membrane perforation. Taken together, these results provided new insights into the molecular mechanism of B. cereus CLO-mediated cytotoxicity.
DOI	10.1038/s41420-024-01887-7
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:001181435500001
出版者	SPRINGERNATURE
WOS关键词	TOXIN PERFRINGOLYSIN-O ; GASDERMIN D ; INFLAMMATORY CASPASES ; NLRP3 INFLAMMASOME ; NALP3 INFLAMMASOME ; OXIDATIVE STRESS ; PATHOGENICITY ; ACTIVATION ; CYTOLYSINS ; GSDMD
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184823
专题	实验海洋生物学重点实验室
通讯作者	Zhao, Yan; Sun, Li
作者单位	1.Inst Oceanol, Chinese Acad Sci, CAS Ctr Ocean Mega Sci, CAS, Qingdao, Peoples R China 2.Chinese Acad Sci, CAS Ctr Ocean Mega Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao, Peoples R China 5.Tsinghua Univ, Sch Med, Tsinghua Peking Joint Ctr Life Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Yujian,Luo, Jingchang,Guan, Xiaolu,et al. Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner[J]. CELL DEATH DISCOVERY,2024,10(1):9.
APA	Wang, Yujian,Luo, Jingchang,Guan, Xiaolu,Zhao, Yan,&Sun, Li.(2024).Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner.CELL DEATH DISCOVERY,10(1),9.
MLA	Wang, Yujian,et al."Bacillus cereus cereolysin O induces pyroptosis in an undecapeptide-dependent manner".CELL DEATH DISCOVERY 10.1(2024):9.