Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens

doi:10.1021/acs.jafc.4c00573

IOCAS-IR > 实验海洋生物学重点实验室

	Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens
	Wei, Xiaotong 1; Hu, Yuanyuan 1; Sun, Chaomin 2,3,4; Wu, Shimei 1
	2024-03-01
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
卷号	72 期号:10 页码:5283-5292
通讯作者	Wu, Shimei(shimeiwu2016@126.com)
摘要	The increasing emergence of multidrug-resistant pathogens and development of biopreservatives in food industries has increased the demand of novel and safe antimicrobial agents. In this study, a marine bacterial strain Bacillus licheniformis M1 was isolated and exhibited obvious antimicrobial activities against foodborne pathogens, especially against methicillin-resistant Staphylococcus aureus. The antimicrobial agent was purified and identified as a novel antimicrobial peptide, which was designated as bacipeptin, and the corresponding mechanism was further investigated by electron microscopy observation and transcriptomic analysis with biochemical validation. The results showed that bacipeptin could reduce the virulence of methicillin-resistant Staphylococcus aureus and exerted its antimicrobial activity by interfering with histidine metabolism, inducing the accumulation of reactive oxygen species and down-regulating genes related to Na+/H+ antiporter and the cell wall, thus causing damage to the cell wall and membrane. Overall, our study provides a novel natural product against foodborne pathogens and discloses the corresponding action mechanism.
关键词	antimicrobial peptides foodborne pathogen Bacillus transcriptomic analysis antimicrobialmechanism
DOI	10.1021/acs.jafc.4c00573
收录类别	SCI
语种	英语
资助项目	Science and Technology Innovation Project of Laosh an Laboratory[LSKJ202203103] ; Science and Technology Innovation Project of Laosh an Laboratory[2022QNLM030004-3] ; NSFC Innovative Group Grant[42221005] ; Shandong Provincial Natural Science Foundation[ZR2021MD099] ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; Major Research Plan of the National Natural Science Foundation[92351301] ; Taishan Scholars Program[tstp20230637]
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:001179315000001
出版者	AMER CHEMICAL SOC
WOS关键词	NA+/H+ ANTIPORTER ; STAPHYLOCOCCUS-AUREUS ; HISTIDINE ; FOOD ; PREVALENCE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184867
专题	实验海洋生物学重点实验室
通讯作者	Wu, Shimei
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wei, Xiaotong,Hu, Yuanyuan,Sun, Chaomin,et al. Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(10):5283-5292.
APA	Wei, Xiaotong,Hu, Yuanyuan,Sun, Chaomin,&Wu, Shimei.(2024).Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(10),5283-5292.
MLA	Wei, Xiaotong,et al."Characterization of a Novel Antimicrobial Peptide Bacipeptin against Foodborne Pathogens".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.10(2024):5283-5292.