Dietary steroids promote body weight growth and induce gametogenesis by increasing the expressions of genes related to cell proliferation of sea cucumber (Apostichopus japonicus)

doi:10.1016/j.cbd.2024.101191

	Dietary steroids promote body weight growth and induce gametogenesis by increasing the expressions of genes related to cell proliferation of sea cucumber (Apostichopus japonicus)
	Liang, Wen-ke 1,2,3,4,5; Zhang, Li-bin 2,3,4,5,7; Xu, Jia-lei 6
	2024-03-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN	1744-117X
卷号	49 页码:11
通讯作者	Zhang, Li-bin(zhanglibin@qdio.ac.cn)
摘要	Steroids play a vital role in animal survival, promoting growth and development when administered appropriate concentration exogenously. However, it remains unclear whether steroids can induce gonadal development and the underlying mechanism. This study assessed sea cucumber weights post-culturing, employing paraffin sections and RNA sequencing (RNA-seq) to explore gonadal changes and gene expression in response to exogenous steroid addition. Testosterone and cholesterol, dissolved in absolute ethanol, were incorporated into sea cucumber diets. After 30 days, testosterone and cholesterol significantly increased sea cucumber weights, with the total weight of experimental groups surpassing the control. The testosterone-fed group exhibited significantly higher eviscerated weight than the control group. In addition, dietary steroids influenced gonad morphology and upregulated genes related to cell proliferation,such as RPL35, PC, eLF-1, MPC2, ADCY10 and CYP2C18. Thees upregulated differentially expressed genes were significantly enriched in the organic system, metabolism, genetic information and environmental information categories. These findings imply that steroids may contribute to the growth and the process of genetic information translation and protein synthesis essential for gonadal development and gametogenesis.
关键词	Steroids Testosterone Cholesterol Apostichopus japonicus RNA -sequencing
DOI	10.1016/j.cbd.2024.101191
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China (NSFC-FDCT project)[42061160365] ; Natural science Foundation of Shandong Province[ZR2023MD090]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:001170350200001
出版者	ELSEVIER SCIENCE INC
WOS关键词	CHOLESTEROL REQUIREMENT ; CYTOCHROME-P450 GENES ; REPRODUCTIVE-BIOLOGY ; TELEOST FISHES ; JAPANESE EEL ; TESTOSTERONE ; HORMONE ; IDENTIFICATION ; METABOLISM ; PRAWN
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184903
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Li-bin
作者单位	1.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 6.Zhongke Tonhe Shandong Marine Technol Co Ltd, Dongying 257200, Peoples R China 7.Inst Oceanol, Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liang, Wen-ke,Zhang, Li-bin,Xu, Jia-lei. Dietary steroids promote body weight growth and induce gametogenesis by increasing the expressions of genes related to cell proliferation of sea cucumber (Apostichopus japonicus)[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2024,49:11.
APA	Liang, Wen-ke,Zhang, Li-bin,&Xu, Jia-lei.(2024).Dietary steroids promote body weight growth and induce gametogenesis by increasing the expressions of genes related to cell proliferation of sea cucumber (Apostichopus japonicus).COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,49,11.
MLA	Liang, Wen-ke,et al."Dietary steroids promote body weight growth and induce gametogenesis by increasing the expressions of genes related to cell proliferation of sea cucumber (Apostichopus japonicus)".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 49(2024):11.