Structure and function analyses of the SRC gene in Pacific white shrimp<i> Litopenaeus vannamei</i>

doi:10.1016/j.fsi.2024.109433

IOCAS-IR > 实验海洋生物学重点实验室

	Structure and function analyses of the SRC gene in Pacific white shrimp Litopenaeus vannamei
	Si, Shuqing 1,2,3; Zhang, Xiaojun 1,2,3; Yu, Yang1,2,3 ; Zhong, Xiaoyun 1,2,3; Zhang, Xiaoxi1,2 ; Yuan, Jianbo 1,2,3; Li, Fuhua 1,2,3,4
	2024-04-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	147 页码:12
通讯作者	Zhang, Xiaojun(xjzhang@qdio.ac.cn)
摘要	SRC gene encodes scavenger receptor class C, a member of the scavenger receptor family, and has only been identified and investigated in invertebrates. Our previous studies have revealed that SRC is a novel candidate gene associated with body weight in Pacific white shrimp (Litopenaeus vannamei). In order to comprehend the underlying mechanism by which LvSRC affects shrimp growth, we analyzed the structure, phylogeny, expression profiles and RNA interference (RNAi) of this gene in L. vannamei. We found that LvSRC contains two CCP domains and one MAM domain, with the highest expression level in the heart and relatively low expression level in other tissues. Notably, LvSRC exhibited significantly higher expression levels in the fast-growing group among groups with different growth rates, suggesting its potential involvement as a gene contributing to the growth of L. vannamei. RNAi of LvSRC inhibited body length and body weight gain compared to the control groups. Moreover, through RNA-seq analysis, we identified 598 differentially expressed genes (DEGs), including genes associated with growth, immunity, protein processing and modification, signal transduction, lipid synthesis and metabolism. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed significant changes in the signaling pathways related to growth, lipid metabolism and immune response, suggesting that LvSRC exhibits the potential to participate in diverse physiological processes and immune regulation. These findings deepen our understanding of the structure and function of the SRC in shrimp and lay the foundation for further research into the regulatory mechanism of LvSRC. Additionally, they provide potential applications in shrimp genetics and breeding.
关键词	Litopenaeus vannamei SRC Gene structure and function Gene expression Growth
DOI	10.1016/j.fsi.2024.109433
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105] ; National Natural Sciences Foundation of China[32273102] ; National Natural Sciences Foundation of China[31972782] ; National Key R & D Program of China[2022YFF1000304] ; Agriculture Research System -48 of China[CARS -48] ; Taishan Scholars Program ; Oceanographic Data Center, IOCAS
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:001187940300001
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS关键词	SCAVENGER RECEPTORS ; MARSUPENAEUS-JAPONICUS ; INNATE IMMUNITY ; PROTEIN ; EXPRESSION ; PHAGOCYTOSIS ; INNEXIN-2 ; SEQUENCE ; GROWTH ; DOMAIN
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184999
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Xiaojun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Si, Shuqing,Zhang, Xiaojun,Yu, Yang,et al. Structure and function analyses of the SRC gene in Pacific white shrimp Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2024,147:12.
APA	Si, Shuqing.,Zhang, Xiaojun.,Yu, Yang.,Zhong, Xiaoyun.,Zhang, Xiaoxi.,...&Li, Fuhua.(2024).Structure and function analyses of the SRC gene in Pacific white shrimp Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,147,12.
MLA	Si, Shuqing,et al."Structure and function analyses of the SRC gene in Pacific white shrimp Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 147(2024):12.