HSP70A promotes the photosynthetic activity of marine diatom <i>Phaeodactylum tricornutum</i> under high temperature

doi:10.1111/tpj.16730

IOCAS-IR > 实验海洋生物学重点实验室

	*HSP70A promotes the photosynthetic activity of marine diatom Phaeodactylum tricornutum* under high temperature**
	Yang, Wenting 1,2,3; Gao, Shan 2,3,4; Bao, Mengjiao 2,3,5; Li, Xin2,3,4,6 ; Liu, Zhiyuan 1; Wang, Guangce 2,3,4
	2024-03-25
发表期刊	PLANT JOURNAL
ISSN	0960-7412
页码	9
通讯作者	Liu, Zhiyuan(liuzhiyuan111@163.com) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	With global climate change, the high-temperature environment has severely impacted the community structure and phenotype of marine diatoms. Phaeodactylum tricornutum, a model species of marine diatom, is sensitive to high temperature, which grow slowly under high temperature. However, the regulatory mechanism of P. tricornutum in response to high-temperature is still unclear. In this study, we found that the expression level of the HSP70A in the wild type (WT) increased 28 times when exposed to high temperature (26(degrees)C) for 1 h, indicating that HSP70A plays a role in high temperature in P. tricornutum. Furthermore, overexpression and interference of HSP70A have great impact on the exponential growth phase of P. tricornutum under 26(degrees)C. Moreover, the results of Co-immunoprecipitation (Co-IP) suggested that HSP70A potentially involved in the correct folding of the photosynthetic system-related proteins (D1/D2), preventing aggregation. The photosynthetic activity results demonstrated that overexpression of HSP70A improves non-photochemical quenching (NPQ) activity under high-temperature stress. These results reveal that HSP70A regulates the photosynthetic activity of P. tricornutum under high temperatures. This study not only helps us to understand the photosynthetic activity of marine diatoms to high temperature but also provides a molecular mechanism for HSP70A in P. tricornutum under high-temperature stress.
关键词	Phaeodactylum tricornutum HSP70A high-temperature stress photosynthetic activity
DOI	10.1111/tpj.16730
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China ; Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; China Agriculture Research System of MOF[CARS-50] ; [42376093] ; [42276146]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:001189987800001
出版者	WILEY
WOS关键词	SYNTHESIS ELONGATION-FACTOR ; HEAT-SHOCK PROTEINS ; EF-TU ; MOLECULAR CHAPERONES ; STRESS TOLERANCE ; PHOTOSYSTEM-II ; EXPRESSION ; TRANSFORMATION ; EVOLUTIONARY ; IMPORT
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185062
专题	实验海洋生物学重点实验室
通讯作者	Liu, Zhiyuan; Wang, Guangce
作者单位	1.Hainan Univ, Sch Marine Biol & Fisheries, Haikou, Hainan, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yang, Wenting,Gao, Shan,Bao, Mengjiao,et al. HSP70A promotes the photosynthetic activity of marine diatom Phaeodactylum tricornutum under high temperature[J]. PLANT JOURNAL,2024:9.
APA	Yang, Wenting,Gao, Shan,Bao, Mengjiao,Li, Xin,Liu, Zhiyuan,&Wang, Guangce.(2024).HSP70A promotes the photosynthetic activity of marine diatom Phaeodactylum tricornutum under high temperature.PLANT JOURNAL,9.
MLA	Yang, Wenting,et al."HSP70A promotes the photosynthetic activity of marine diatom Phaeodactylum tricornutum under high temperature".PLANT JOURNAL (2024):9.