Microeukaryotes have unexpected importance in cold seep food webs through predation and parasitism

doi:10.1016/j.pocean.2024.103216

	Microeukaryotes have unexpected importance in cold seep food webs through predation and parasitism
	Li, Longzhao 1,2,3; Zhao, Feng 1,2,3,5; Filker, Sabine 4; Liu, Weiyue 1,3; Zhao, Rongjie1 ; Wan, Yuanyuan 1,3; Xu, Kuidong1,2,3,5
	2024-03-01
发表期刊	PROGRESS IN OCEANOGRAPHY
ISSN	0079-6611
卷号	222 页码:13
通讯作者	Zhao, Feng(fzhao@qdio.ac.cn) ; Xu, Kuidong(kxu@qdio.ac.cn)
摘要	Cold seeps are inhabited by a high abundance of chemosynthetic prokaryotes and invertebrates. However, a complete food web between invertebrates and prokaryotes cannot be established without the important link of microeukaryotes. Yet, question such as which microeukaryotes are at work in cold seeps is still mostly open. We, therefore, compared the biological communities in cold seeps and adjacent areas of the South China Sea by the metabarcoding and quantitative PCR (qPCR) of nuclear 18S and 16S rRNA genes, and estimated the potentially ecological roles of microeukaryotes by cross-domain microbial network analysis. The anaerobic methane oxidizing archaea (ANME), sulfate-reducing bacteria (SRB) and Radiolaria accounted for most variations between microbial communities from the cold seeps and adjacent areas. A significant correlation was detected between the copy numbers of 18S rRNA gene and communities of the ANME and SRB in cold seeps, indicating close links between microeukaryotes and chemosynthetic prokaryotes. After adding microeukaryotic nodes microbial network became more complex, and the negative correlation proportion and average path length increased, suggesting increased predation and parasitism. The ANME and SRB groups were mainly negatively correlated with and potentially preyed by Acantharea and Cercozoa, which were then negatively correlated with annelids and mollusks. Parasitic microeukaryotes of Syndiniales widely existed in cold seeps and had close relationships with Polycystinea. Parasitism might cause the death of Polycystinea and the release of Syndiniales spores, which may be an important carbon source for the cold seep biota. Using Mantel analyses, we also demonstrated that trophic relationships are closer in cold seeps than in the adjacently deep-sea sediments, and that microeukaryote-metazoans linkages in cold seeps are closer than those between prokaryotes and metazoans. Overall, our study outlines the main components of food webs in cold seeps and highlights that microeukaryotes have an unexpected importance in the carbon cycle.
关键词	Cold seeps Eukaryotes Prokaryotes Cross-domain networks Bio-interactions qPCR
DOI	10.1016/j.pocean.2024.103216
收录类别	SCI
语种	英语
资助项目	Science and Tech-nology Innovation Project of Laoshan Laboratory[LSKJ202203102] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050203] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; Youth Innovation Promotion Association CAS[2022206]
WOS研究方向	Oceanography
WOS类目	Oceanography
WOS记录号	WOS:001201734200001
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS关键词	DIVERSITY ; METHANE ; COMMUNITY ; OXIDATION ; SEDIMENTS ; NETWORKS ; PATTERNS ; PROTISTS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185137
专题	海洋生物分类与系统演化实验室
通讯作者	Zhao, Feng; Xu, Kuidong
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao Key Lab Marine Biodivers & Conservat, Qingdao 266071, Peoples R China 2.Laoshan Lab, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Univ Kaiserslautern Landau, Dept Mol Ecol, D-67663 Kaiserslautern, Germany 5.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Longzhao,Zhao, Feng,Filker, Sabine,et al. Microeukaryotes have unexpected importance in cold seep food webs through predation and parasitism[J]. PROGRESS IN OCEANOGRAPHY,2024,222:13.
APA	Li, Longzhao.,Zhao, Feng.,Filker, Sabine.,Liu, Weiyue.,Zhao, Rongjie.,...&Xu, Kuidong.(2024).Microeukaryotes have unexpected importance in cold seep food webs through predation and parasitism.PROGRESS IN OCEANOGRAPHY,222,13.
MLA	Li, Longzhao,et al."Microeukaryotes have unexpected importance in cold seep food webs through predation and parasitism".PROGRESS IN OCEANOGRAPHY 222(2024):13.