A study on the effect of temperature training on compensatory growth and pathogen resistance of post-larval <i>Litopenaeus vannamei</i>

doi:10.1007/s10499-024-01520-5

IOCAS-IR > 实验海洋生物学重点实验室

	A study on the effect of temperature training on compensatory growth and pathogen resistance of post-larval Litopenaeus vannamei
	Zhao, Zhen1,2,4 ; Liu, Yuan 1,2; Wang, Baojie 1,2; Jiang, Keyong 1,2; Xu, Kefeng 3; Zhong, Chen 3; Gao, Yan3 ; Liu, Mei 3; Wang, Lei 1,2,4
	2024-04-22
发表期刊	AQUACULTURE INTERNATIONAL
ISSN	0967-6120
页码	25
通讯作者	Liu, Mei(mliu_msrisd@126.com) ; Wang, Lei(leiwang@qdio.ac.cn)
摘要	The technology of post-larval farming is increasingly being valued by aquaculture farmers and enterprises. The objective of this study is to determine an appropriate temperature restriction mode through temperature training to achieve greater compensatory growth potential. We cultured PL whiteleg shrimp (Litopenaeus vannamei) at either low (25degree celsius) or high (31degree celsius) temperature for 20 days (restriction stage) and then transferred them to a control temperature (28degree celsius) for 20 days (compensation stage). The compensatory growth observed under low temperature restriction showed that shrimp can recover and exhibit over-compensatory growth after growth restriction. Specific growth ratio and enzyme activity analyses indicated that the shrimp required 10 days or more to adapt to temperature stress. The relative expression levels of hsp60, tor, and s6k in the low temperature group were downregulated during the early stage of the restriction period, but were continually upregulated during the compensation period. This result indicates that although short-term restrictions may lead to growth retardation, growth will resume as the temperature recovers. When shrimp were challenged with Vibrio parahaemolyticus E1, the damage caused by infection after low temperature restriction and compensation was delayed and not severe. This indicates that the disease resistance of shrimp was improved after low temperature restriction. In contrast, shrimp in the high temperature group showed more severe damage than those in the control group at all time points after pathogen infection, indicating that high temperature restriction made the shrimp more susceptible to pathogenic Vibrio. Transcriptome analysis revealed abundant pathways related to energy metabolism, amino acid biosynthesis, protein synthesis, and cell growth in the low temperature restriction-recovery group, which highlighted the importance of optimizing the nutrient supply, especially protein and amino acid to enhance compensatory growth. In summary, after 20 days of low temperature training, PL shrimp exhibited significant compensatory growth, which not only promoted the growth, but also enhanced the body's resistance to V. parahaemolyticus. Therefore, this culture technique can be used for aquaculture practices in PL shrimp cultivation.
关键词	Litopenaeus vannamei Temperature restriction Compensatory growth Transcriptomics Pathogen resistance Adaptive response
DOI	10.1007/s10499-024-01520-5
收录类别	SCI
语种	英语
资助项目	Natural Science Foundation of Shandong Province
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:001206256600001
出版者	SPRINGER
WOS关键词	JUVENILE CHINESE SHRIMP ; EXPRESSION ; GENE ; AQUACULTURE ; IMMUNITY ; MEMORY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185571
专题	实验海洋生物学重点实验室
通讯作者	Liu, Mei; Wang, Lei
作者单位	1.Chinese Acad Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Marine Sci Res Inst Shandong Prov, Qingdao Aquat Organisms Qual Evaluat & Utilizat En, Shandong Key Lab Dis Control Mariculture, 7 Youyun Rd, Qingdao 266104, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhao, Zhen,Liu, Yuan,Wang, Baojie,et al. A study on the effect of temperature training on compensatory growth and pathogen resistance of post-larval Litopenaeus vannamei[J]. AQUACULTURE INTERNATIONAL,2024:25.
APA	Zhao, Zhen.,Liu, Yuan.,Wang, Baojie.,Jiang, Keyong.,Xu, Kefeng.,...&Wang, Lei.(2024).A study on the effect of temperature training on compensatory growth and pathogen resistance of post-larval Litopenaeus vannamei.AQUACULTURE INTERNATIONAL,25.
MLA	Zhao, Zhen,et al."A study on the effect of temperature training on compensatory growth and pathogen resistance of post-larval Litopenaeus vannamei".AQUACULTURE INTERNATIONAL (2024):25.