Effects of hypo-osmotic stress on osmoregulation, antioxidant response, and energy metabolism in sea cucumber Holothuria moebii under desalination environment

doi:10.1016/j.envres.2024.118800

	Effects of hypo-osmotic stress on osmoregulation, antioxidant response, and energy metabolism in sea cucumber Holothuria moebii under desalination environment
	Jiang, Junyang 1; Tang, Yanna 1; Cao, Zhaozhao 1; Zhou, Cong2 ; Yu, Zonghe 1
	2024-07-01
发表期刊	ENVIRONMENTAL RESEARCH
ISSN	0013-9351
卷号	252 页码:15
通讯作者	Zhou, Cong(zhoucong@qdio.ac.cn) ; Yu, Zonghe(yuzh@scau.edu.cn)
摘要	With global climate changing, hypo -salinity events are increasing in frequency and duration because of continuous rainfall and freshwater inflow, which causes reduced cytosolic osmolarity and cellular stress responses in aquatic animals. Sea cucumbers are considered stenohaline because they lack osmoregulatory organs and are vulnerable to salinity fluctuations. In this study, we performed multiple biochemical assays, de novo transcriptomics, and widely targeted metabolomics to comprehensively explore the osmoregulatory mechanisms and physiological responses of sea cucumber Holothuria moebii to hypo -osmotic stress, which is a representative specie that is frequently exposed to hypo -saline intertidal zones. Our results found that H . moebii contracted their ambulacral feet and oral tentacles, and the coelomic fluid ion concentrations were reduced to be consistent with the environment. The microvilli of intestines and respiratory trees underwent degeneration, and the cytoplasm exhibited swelling and vacuolation. Moreover, the Na + , K + , and Cl - concentrations and Na + /K + -ATPase activity were significantly reduced under hypo -osmotic stress. The decrease in protein kinase A activity and increase in 5 ' -AMP level indicated a significant inhibition of the cAMP signaling pathway to regulate ion concentrations. And small intracellular organic molecules (amino acids, nucleotides and their derivatives) also play crucial roles in osmoregulation through oxidative deamination of glutamate, nucleotide catabolism, and nucleic acid synthesis. Moreover, lysosomes and peroxisomes removed oxidative damage, whereas antioxidant metabolites, such as Nacetyl amino acids and glutathione, were increased to resist oxidative stress. With prolonged hypo -osmotic stress, glycerophospholipid metabolism was enhanced to maintain membrane stability. Furthermore, acyl-CoA-binding protein activity was significantly inhibited, and only a small amount of acylcarnitine was significantly accumulated, which indicated a disruption in energy metabolism. PPAR signaling pathway and choline content were up -regulated to promote fatty acid metabolism under hypo -osmotic stress. Overall, our results provide new insights into the osmoregulatory mechanisms and physiological responses of sea cucumbers to hypo -osmotic stress.
关键词	Holothurian Hypo-osmotic stress Osmoregulation Oxidative stress Energy metabolism
DOI	10.1016/j.envres.2024.118800
收录类别	SCI
语种	英语
资助项目	National Key R & D Program of China[2022YFD2401303] ; Guangdong Province University Students' Science and Technology Innovation Cultivation Special Fund[pdjh2023a0076]
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS记录号	WOS:001225463400001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS关键词	ECHINOMETRA-LUCUNTER ECHINODERMATA ; APOSTICHOPUS-JAPONICUS ; VOLUME REGULATION ; OXIDATIVE STRESS ; AMINO-ACIDS ; ACYL-COA ; PHYSIOLOGICAL PERFORMANCE ; PYRIDINE-NUCLEOTIDES ; GENE-EXPRESSION ; SMALL MOLECULES
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185734
专题	海洋生态与环境科学重点实验室
通讯作者	Zhou, Cong; Yu, Zonghe
作者单位	1.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Jiang, Junyang,Tang, Yanna,Cao, Zhaozhao,et al. Effects of hypo-osmotic stress on osmoregulation, antioxidant response, and energy metabolism in sea cucumber Holothuria moebii under desalination environment[J]. ENVIRONMENTAL RESEARCH,2024,252:15.
APA	Jiang, Junyang,Tang, Yanna,Cao, Zhaozhao,Zhou, Cong,&Yu, Zonghe.(2024).Effects of hypo-osmotic stress on osmoregulation, antioxidant response, and energy metabolism in sea cucumber Holothuria moebii under desalination environment.ENVIRONMENTAL RESEARCH,252,15.
MLA	Jiang, Junyang,et al."Effects of hypo-osmotic stress on osmoregulation, antioxidant response, and energy metabolism in sea cucumber Holothuria moebii under desalination environment".ENVIRONMENTAL RESEARCH 252(2024):15.