Transcriptomic insights into vibrio-induced mortality in the clam Meretrix petechialis under high temperature

doi:10.1016/j.cbd.2024.101226

IOCAS-IR > 实验海洋生物学重点实验室

	Transcriptomic insights into vibrio-induced mortality in the clam Meretrix petechialis under high temperature
	Dong, Li 1,2; Yu, Jiajia2 ; Wang, Hongxia 2; Yue, Xin 2; Liu, Baozhong2,3
	2024-06-01
发表期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
ISSN	1744-117X
卷号	50 页码:9
通讯作者	Wang, Hongxia(hxwang@qdio.ac.cn) ; Yue, Xin(yuexin@qdio.ac.cn)
摘要	In this study, we investigate the mortality of the clam Meretrix petechialis facing a vibrio challenge under different temperatures and the underlying molecular mechanisms. Our experiment distinctly revealed that clam mortality was predominantly observed under high temperature, highlighting the critical impact of thermal stress on clam susceptibility to infection. Using RNA-seq, we further compared the global transcriptional response to vibrio in clam gills between high and low temperatures. Compared to other groups, the differentially expressed genes in vibrio-challenged group at high temperature associated with immunity, oxidative stress, and membrane transport. Key results show a weakened immune response in clams at high temperature, especially in the TNF signaling pathway, and a decrease in membrane transport efficiency, notably in SLC proteins. Additionally, high temperature enhanced pro-inflammatory related unsaturated fatty acid metabolism, leading to increased oxidative damage. This was further evidenced by our biochemical assays, which showed significantly higher levels of lipid peroxidation and protein carbonylation in clams at high temperature, indicating heightened oxidative damage. RT-PCR validation of selected DEGs corroborated the RNA-seq findings. Our findings contribute to the understanding of more frequent shellfish mortality in summer, emphasizing the role of temperature in pathogen response, elucidating the molecular mechanisms underlying the synergistic effect of pathogen and high temperature stresses. The key genes identified provide potential targets for resistance-assisted breeding. This research has significant implications for bivalve aquaculture and their physiology, particularly in light of global climate changes affecting marine ecosystems.
关键词	Bivalve Thermally -induced epidemic Immunity Membrane transport Oxidative damage
DOI	10.1016/j.cbd.2024.101226
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2023YFD2400800] ; Shandong Province Natural Science Foundation (China)[ZR2020MD089] ; Shandong Province Natural Science Foundation (China)[CARS-49] ; Open Project of CAS Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture[2023FB06] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; Key Research and Development Program of Shandong Province[2021LZGC029] ; Taishan Industrial Leading Talents Project
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:001223395800001
出版者	ELSEVIER SCIENCE INC
WOS关键词	LITOPENAEUS-VANNAMEI ; STRESS ; IDENTIFICATION ; INFLAMMATION ; INFECTION ; SURVIVAL ; IMMUNITY ; DISEASE ; LACCASE ; GROWTH
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/185755
专题	实验海洋生物学重点实验室
通讯作者	Wang, Hongxia; Yue, Xin
作者单位	1.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Dong, Li,Yu, Jiajia,Wang, Hongxia,et al. Transcriptomic insights into vibrio-induced mortality in the clam Meretrix petechialis under high temperature[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2024,50:9.
APA	Dong, Li,Yu, Jiajia,Wang, Hongxia,Yue, Xin,&Liu, Baozhong.(2024).Transcriptomic insights into vibrio-induced mortality in the clam Meretrix petechialis under high temperature.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,50,9.
MLA	Dong, Li,et al."Transcriptomic insights into vibrio-induced mortality in the clam Meretrix petechialis under high temperature".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 50(2024):9.